LANCE Ultra phospho-STAT3 (Tyr705) kits are designed for the detection of phosphorylated STAT3 in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Please note control lysates are NOT included in the kits. Control lysates are sold separately, catalog number TRF4004S.
Formats:
LANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).
STAT3 is an important transcriptional activator, and is involved in regulating cytokines and growth factor receptors. STAT3 is phosphorylated at Tyr-705 by the JAK pathway, upon which STAT3 will dimerize, translocate to the nucleus, and bind DNA. In some cancers STAT3 is shown to be constitutively activated. It has been implicated in both anti-apoptotic and oncogenic pathways.
Application |
Kinase
Protein Analysis & Detection
|
---|---|
Assay Pathway |
JAK/STAT
|
Assay Points |
10000
|
Assay Target Class |
Phospho-protein
|
Automation Compatible |
Yes
|
Brand |
LANCE Ultra
|
Detection Method |
Time-Resolved Fluorescence (TRF)
TR-FRET
|
Shipping Conditions |
Shipped in Blue Ice
|
Therapeutic Area |
Autoimmunity
Neuroscience
Respiratory
|
Unit Size |
10,000 Assay Points
|
Emerging pathways to neuroinflammation and neurodegeneration
Neurodegenerative diseases, such as amyotrophic lateral sclerosis...
STAT3 is a particularly important therapeutic target since it is constitutively active in a range of cancer cells lines and human...
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