LANCE Ultra total SLP-76 kits are designed for the detection of SLP-76 (phosphorylated and non-phosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay can be used as a normalizaton assay for phospho-SLP-76, and is compatible with both adherent and suspension cells.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Please note control lysates are NOT included in kits. Control lysates are sold separately, catalog number TRF4010S.
Formats:
LANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).
Application |
Kinase
Protein Analysis & Detection
|
---|---|
Assay Points |
500
|
Assay Target Class |
Phospho-protein
|
Automation Compatible |
Yes
|
Brand |
LANCE Ultra
|
Detection Method |
TR-FRET
|
Shipping Conditions |
Shipped in Blue Ice
|
Therapeutic Area |
Autoimmunity
Immuno-oncology
Inflammation
Neuroscience
Oncology
|
Unit Size |
500 Assay Points
|
STAT3 is a particularly important therapeutic target since it is constitutively active in a range of cancer cells lines and human...
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