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LANCE Ultra Total SLP76 Detection Kit, 10,000 Assay Points

LANCE Ultra total SLP-76 kits are designed for the detection of SLP-76 (phosphorylated and non-phosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay can be used as a normalizaton assay for phospho-SLP-76, and is compatible with both adherent and suspension cells.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Feature Specification
Application Cell Signaling
Sample Volume 15 µL

LANCE Ultra total SLP-76 kits are designed for the detection of SLP-76 (phosphorylated and non-phosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay can be used as a normalizaton assay for phospho-SLP-76, and is compatible with both adherent and suspension cells.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Unit Size: 500 Assay Points
Part #:
TRF4017C
List Price
USD 2,043.00
Unit Size: 10,000 Assay Points
Part #:
TRF4017M
List Price
USD 9,232.00

Overview

Please note control lysates are NOT included in kits. Control lysates are sold separately, catalog number TRF4010S.

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 384-well format, using a 20 µL reaction volume.
  • Our 10,000 assay point kit allows you to run 10,000 wells in 384-well format, using a 20 µL reaction volume.

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).

Specifications

Application
Cell Signaling
Automation Compatible
Yes
Brand
LANCE Ultra
Detection Modality
LANCE
Molecular Modification
Total
Product Group
Kit
Sample Volume
15 µL
Shipping Conditions
Shipped in Blue Ice
Target
SLP-76
Target Class
Phosphoproteins
Technology
TR-FRET
Therapeutic Area
Autoimmunity
Immuno-oncology
Inflammation
Neuroscience
Oncology
Unit Size
10,000 Assay Points

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