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LANCE Ultra Total SLP76 Detection Kit, 10,000 Assay Points
LANCE Ultra Total SLP76 Detection Kit, 10,000 Assay Points
LANCE Ultra total SLP-76 kits are designed for the detection of SLP-76 (phosphorylated and non-phosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay can be used as a normalizaton assay for phospho-SLP-76, and is compatible with both adherent and suspension cells.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
| Feature | Specification |
|---|---|
| Application | Cell Signaling |
| Sample Volume | 15 µL |
LANCE Ultra total SLP-76 kits are designed for the detection of SLP-76 (phosphorylated and non-phosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay can be used as a normalizaton assay for phospho-SLP-76, and is compatible with both adherent and suspension cells.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
LANCE Ultra Total SLP76 Detection Kit, 10,000 Assay Points
LANCE Ultra Total SLP76 Detection Kit, 10,000 Assay Points
Product information
Overview
Please note control lysates are NOT included in kits. Control lysates are sold separately, catalog number TRF4010S.
Formats:
- Our 500 assay point kit allows you to run 500 wells in 384-well format, using a 20 µL reaction volume.
- Our 10,000 assay point kit allows you to run 10,000 wells in 384-well format, using a 20 µL reaction volume.
LANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).
Specifications
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
LANCE Ultra
|
| Detection Modality |
LANCE
|
| Molecular Modification |
Total
|
| Product Group |
Kit
|
| Sample Volume |
15 µL
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
SLP-76
|
| Target Class |
Phosphoproteins
|
| Technology |
TR-FRET
|
| Therapeutic Area |
Autoimmunity
Immuno-oncology
Inflammation
Neuroscience
Oncology
|
| Unit Size |
10,000 Assay Points
|
Resources
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LANCE™ Ultra TR-FRET is a no-wash proximity assay technology that combines the benefits of time resolution (TR) with fluorescence...
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