LANCE Ultra phospho-ZAP-70 (Tyr319) kits are designed for the detection of phosphorylated ZAP-70 in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Please note control lysates are sold separately, catalog number TRF4024S.
Formats:
LANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).
ZAP-70 (Zeta-chain-associated protein kinase 70) is normally expressed near the surface membrane of T-cells and natural killer cells, but levels in B-cells can be an indicator for various forms of leukemia. ZAP-70 is recruited to the TCR/CD3 complex in order to be phosphorylated. Once phosphorylated, ZAP-70 promotes downstream phosphorylation of proteins such as SLP76 and ERK.
Application |
Kinase
Protein Analysis & Detection
|
---|---|
Assay Points |
10000
|
Assay Target Class |
Phospho-protein
|
Automation Compatible |
Yes
|
Brand |
LANCE Ultra
|
Detection Method |
Time-Resolved Fluorescence (TRF)
TR-FRET
|
Shipping Conditions |
Shipped in Blue Ice
|
Therapeutic Area |
Autoimmunity
Immuno-oncology
Oncology
|
Unit Size |
10,000 Assay Points
|
STAT3 is a particularly important therapeutic target since it is constitutively active in a range of cancer cells lines and human...
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