The LANCE® Ultra Human tPA Detection Kit is designed for detection and quantitation of human tPA in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
The LANCE® Ultra Human tPA Detection Kit is designed for detection and quantitation of human tPA in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.
Tissue Plasminogen Activator (tPA) is a 59 kDa secreted serine protease that converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. It is synthesized in numerous tissues and is the principal endogenous activator of plasminogen in blood. It plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis that can result in thrombosis or embolism. Rapid fluctuations in tPA concentration can be observed in response to exercise, venous occlusion, alcohol, and drugs, such as anabolic steroids. Individuals who do not show increased tPA activity when exposed to some of these stimuli, may be at risk for deep vein thrombosis.
Application |
Protein Analysis & Detection
|
---|---|
Assay Points |
500
|
Assay Target |
tPA
|
Assay Target Class |
Protein
|
Automation Compatible |
Yes
|
Brand |
LANCE Ultra
|
Detection Method |
TR-FRET
|
Experimental Type |
In vitro
|
Format |
Microplates
|
One Unit Contains |
500.0 assay points
|
Quantity in a Package Amount |
1.0 Units
|
Species |
Human
|
Shipping Conditions |
Shipped in Blue Ice
|
Therapeutic Area |
Cardiovascular
|
Unit Size |
500 Assay Points
|
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