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LANCE Ultra Phospho-eIF2α (Ser51) Detection Kit, 500 Assay Points

LANCE Ultra phospho-eIF2A (Ser51) kits are designed for the detection of eIF2A (phosphorylated at S51) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Feature Specification
Application Cell Signaling
Sample Volume 15 µL

LANCE Ultra phospho-eIF2A (Ser51) kits are designed for the detection of eIF2A (phosphorylated at S51) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Click to copy promo code to clipboard.
SAVE 15% NOW on online orders. Use promo code below.
YEAREND15
Offer valid until 12/15. Terms and conditions apply.
Product Variants
Unit Size: 500 Assay Points
Part #:
TRF4008C
List Price
USD 2,043.00
Unit Size: 10,000 Assay Points
Part #:
TRF4008M
List Price
USD 9,232.00

Overview

Please note control lysates are NOT included in kits. Control lysates are sold separately, catalog number TRF4008S.

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 384-well format, using a 20 µL reaction volume.
  • Our 10,000 assay point kit allows you to run 10,000 wells in 384-well format, using a 20 µL reaction volume.

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).

Eukaryotic initiation factor 2 (eIF2) is a heterotrimer which is involved in the initiation of translation in eukaryotes. EIF2 binds to two other partners, GTP and Met-tRNAi, and forms the 43S pre-initiation complex upon binding the 40S subunit. Under conditions of cellular stress, the alpha subunit can be phosphorylated at S51 to downregulate protein translation.

Specifications

Application
Cell Signaling
Automation Compatible
Yes
Brand
LANCE Ultra
Detection Modality
LANCE
Molecular Modification
Phosphorylation
Product Group
Kit
Sample Volume
15 µL
Shipping Conditions
Shipped in Blue Ice
Target
eIF2α
Target Class
Phosphoproteins
Technology
TR-FRET
Therapeutic Area
Autoimmunity
Unit Size
500 Assay Points

Resources

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Application Note
LANCE Ultra TR-FRET-based detection and modulation of phosphorylated STAT3 levels in human cells.

STAT3 is a particularly important therapeutic target since it is constitutively active in a range of cancer cells lines and human...

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