LANCE Ultra phospho-EGFR (Tyr1068) kits are designed for the detection of EGFR (phosphorylated at residue Y1068) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Please note control lysates are NOT included in kits. Control lysates are sold separately, catalog number TRF4015S.
Formats:
LANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).
The epidermal growth factor receptor (EGFR) is a receptor tyrosine kinase for a number of extracellular ligands in the EGF family. Ligand binding triggers a number of events including dimerization and auto-phosphorylation. Several major signaling cascades are activated by EGFR.
Application |
Kinase
Protein Analysis & Detection
|
---|---|
Assay Points |
10000
|
Assay Target |
EGFR
|
Assay Target Class |
Phospho-protein
|
Automation Compatible |
Yes
|
Brand |
LANCE Ultra
|
Detection Method |
TR-FRET
|
Shipping Conditions |
Shipped in Blue Ice
|
Unit Size |
10,000 Assay Points
|
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