Europium-labeled anti-V5 antibody, for capturing V5-tagged proteins in LANCE® TR-FRET assays. This Europium-labeled reagent can be used to capture V5-tagged proteins and peptides, and can be used in conjunction with LANCE® ULight or SureLight APC reagents to create TR-FRET no-wash assays for protein-protein interaction, protein detection, and enzymatic studies.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Europium-labeled anti-V5 antibody, for capturing V5-tagged proteins in LANCE® TR-FRET assays.
LANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC), etc.]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm (for ULight dye and APC).
Antibody Conjugates |
Anti-V5
|
---|---|
Application |
Protein-protein interactions
|
Automation Compatible |
Yes
|
Brand |
LANCE
|
Detection Method |
Time-Resolved Fluorescence (TRF)
TR-FRET
|
Experimental Type |
In vitro
|
Fluorophore |
Eu-W1024
|
Format |
Microplates
|
Shipping Conditions |
Shipped in Blue Ice
|
Unit Size |
50 µg
|
Here, we demonstrate the utility and benefits of using LANCE Ultra TR-FRET assay technology for identifying and characterizing PD...
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