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LANCE Anti-6His Eu-Antibody, 1 mg

Europium-labeled anti-6X His antibody, for capturing His-tagged proteins in LANCE® TR-FRET assays.

This is a replacement for AD0111.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Feature Specification
Application Protein-Protein Interaction

Europium-labeled anti-6X His antibody, for capturing His-tagged proteins in LANCE® TR-FRET assays.

This is a replacement for AD0111.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Click to copy promo code to clipboard.
SAVE 15% NOW on online orders. Use promo code below.
YEAREND15
Offer valid until 12/15. Terms and conditions apply.
Product Variants
Unit Size: 10 µg
Part #:
AD0402
List Price
USD 337.78
Unit Size: 50 µg
Part #:
AD0401
List Price
USD 1,607.00
Unit Size: 1 mg
Part #:
AD0400
List Price
USD 15,950.00

Overview

Eu-W1024 labeled anti-6xHis antibodies for LANCE® assays. The anti-6xHis antibody is a purified mouse IgG1 monoclonal antibody. It is raised against a polypeptide containing a 6X histidine tag. The antibody has been shown to detect polyhistidine tags localized at the amino- or carboxyl-terminus.

LANCE (Lanthanide chelate excite) and LANCE® Ultra™ are TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC), etc.]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (˜10 nm). This results in the emission of light at 665 nm (for ULight dye and APC).

Specifications

Application
Protein-Protein Interaction
Automation Compatible
Yes
Brand
LANCE Ultra
Conjugates
Anti-6X His
Detection Modality
LANCE
Fluorophore
Eu-W1024
Product Group
Antibody
Shipping Conditions
Shipped in Dry Ice
Target Class
Binding Assay
Technology
TR-FRET
Unit Size
1 mg

Resources

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1-1 of 1 Resources
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Application Note
Developing a LANCE TR-FRET assay for screening PD-1 and PD-L1 binding inhibitors.

Here, we demonstrate the utility and benefits of using LANCE Ultra TR-FRET assay technology for identifying and characterizing PD...

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