LabChip Small RNA Reagent Kit

Product information

Overview

Save Time & Money by Automating your Small RNA Analysis

Performing small RNA sample analysis with the LabChip small RNA assay saves time and money by automating steps for small RNA sizing and relative abundance. Within a minute, the microfluidic LabChip^® capillary electrophoresis technology automatically separates and analyzes small RNA samples. System software automatically analyzes the data and determines fragment size and concentration using an internal ladder and marker calibration standards. Digital data results are immediately available for review or reporting in a virtual gel, electropherogram graph or table summary.

Characterization and QC of Small RNA Molecules and CRISPR/Cas9 gRNA

To ensure the efficacy of small RNA molecules used as therapeutic agents they must be thoroughly analyzed. This can include size-based analysis to validate in-house manufactured small RNAs or externally sourced material and to screen for the presence of chemical modifications or truncations that may affect their properties. The CRISPR/Cas 9 system allows researchers to target and edit specific genes in a precise and directed manner. gRNAs direct the CRISPR/Cas9 complex to target a specific region within a gene.

Additional product information

Revvity's comprehensive, high-throughput quality control solutions ensure the integrity of gRNAs and small RNA molecules for therapeutic applications. The LabChip Small RNA assay assesses the size, purity, and total concentration of these small RNAs in only 45 seconds per sample. With a single reagent prep, 384 samples can be run on one chip, dramatically increasing throughput and productivity.

High assay accuracy and precision: Pre-packaged reagents and standardized assay protocols yield highly accurate and reproducible data
Fast results: Automated analysis within a minute
Minimal sample consumption: Only 2 μL of sample required
Digital data format: Gel-like image, electropherogram, and tabular formats; automated sample alignment, overlay, scaling and zooming features, conveniently share, store and archive data.
Minimal contamination: Uses RNase-free reagents and chips to avoid sample degradation during analysis, automatic wash cycles minimize carry over among samples.
High-throughput: LabChip microchips and reagent kits are designed for multiple measurement up to 2,000 runs that fit modern high-throughput workflows.

The LabChip small RNA assay is compatible with both the LabChip GX Touch nucleic acid analyzer and the LabChip GXII Touch protein characterization system.

microfluidic capillary electrophoresis of small RNA

Figure 1: LabChipSmall RNA workflow diagram. With the LabChip small RNA assay, you can measure up to 384 samples of small RNA molecules in one sample preparation.

electropherogram distinguishes size of small RNA

Figure 2. The LabChip Small RNA assay automatically measures sizing of small RNA molecules, with good accuracy and precision.

This assay is designed to be used with the DNA 5K / RNA / Charge Variant Assay LabChip (product numbers 760435, 760527) to be run on a the LabChip GX or GXII Touch HT instruments. If running the assay on the LabChip GX Touch 24 Nucleic Acid Analyzer, the DNA 5K/RNA Charge Variant Assay LabChip (LabChip GX Touch 24, LabChip GXII Touch 24) should be used.

Small RNA ladder is provided with the kit; however, if additional ladder is needed it can be ordered separately using part number CLS153593.

Specifications

Other Specifications

Analytical Technique	Analysis & Detection DNA/RNA Isolation
Application	DNA/RNA Quantitation and QC
Brand	LabChip GX
Quantity in a Package Amount	1.0 Units
Research Areas	Biotechnology Pharmaceutics
Sample Type	DNA / RNA
Shipping Conditions	Shipped in Dry Ice
Unit Size	1