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LabChip Low pI Charge Variant Analysis
The LabChip® Charge Variant Analysis Assays allow you to efficiently analyze the charge variant profiles of basic and acidic protein samples.
For research use only. Not for use in diagnostic procedures.
Product Variants
Product information
Overview
Increase the speed, efficiency, and reliability of charge variant analysis.
All biotherapeutic protein molecules come with charge heterogeneity sites, known as charge variants. Charge variants affect biological functions, such as binding and stability. Charge variant profiles also change dynamically throughout processes, such as sequence expression, chemical degradation, and post-translational modification. It is important to monitor and screen charge variant profiles of biotherapeutic proteins throughout development and manufacturing process.
Revvity’s LabChip® charge variant analysis assays are comparable to traditional CZE assays (Han, 2011) and can resolve and screen the charge variant profile of samples quickly, reliably, and automatically. With the throughput capability on the LabChip® GXII Touch™ protein characterization system, users can optimize their valuable research time to accelerate discovery and development of new biotherapeutic solutions.
Additional product information
Obtain speed and efficiency of charge variant analysis throughout biotherapeutic development and manufacturing processes.
- Simple and automated operational workflow
- Fast analysis speed: ~60 seconds per sample
- High throughput: 96 samples per experiment
- Minimal sample consumption: 25 μL
- Full pI range coverage: pI 3 – 9, both acidic and basic proteins
- Protein migration time can be adjusted based on running buffer pH
- Good linearity: peak intensity proportional to variant concentration
- Reliable repeatability with C.V. < 2.5 %
Figure 1. Charge variant profile of a negative charged monoclonal antibody (mAb) on the LabChip low pI charge variant assay. Due to a stronger electrophoretic force, acidic variants move toward the anode faster and arrive earlier at the detection window than main variants and basic variants. Acidic, main and basic variants are separated, resolved and labeled in the LabChip charge variant assay. The relative concentration of each variant was also quantified.
Figure 2. On LabChip Low pI Charge Variant Assay, charge variant profiles of bovine serum albumin shift when pH of running buffer change from pH 5.6 to 7.2.
Ordering Information
- Assay: Labchip Low pl Charge Variant Analysis
- Reagent Kit: CLS154134
- LabChip HT: CLS153418
Specifications
| Analytical Technique |
Protein Analysis & Detection
|
|---|---|
| Application |
Protein Analysis
|
| Brand |
LabChip GXII
|
| Research Areas |
Drug Development & Manufacturing
Drug Discovery & Manufacturing
Food & Agriculture
|
| Sample Type |
Protein
|
| Shipping Conditions |
Shipped in Dry Ice
|
| Type |
Low pI (3.0 - 7.0)
|
References
- Han, H., Livingston, E., and Chen, X. (2011) High Throughput Profiling of Charge Heterogeneity in Antibodies by Microchip Electrophoresis Anal. Chem. 83, 21, 8184–8191. doi.org/10.1021/ac201741w.
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