IVISense 750 MAL Fluorescent Self-Quenching Dye (1 mg) (VivoTag)

IVISense 750 MAL Fluorescent Self-Quenching Dye is a thiol (-SH, sulfhydryl) reactive, maleimide-containing NIR fluorochrome for labeling small molecules. Its superior brightness makes it particularly well suited for a wide range of both in vivo and in vitro imaging applications using labeled thiol-containing molecules.

It is optimized for single molecule loading, such that labeling a protein with multiple IVISense Self-Quenching Dye molecules will cause a shift in the absorbance of the dye and reduce the signal. Therefore, only choose IVISense Self-Quenching Dyes if your labeling ratio of dye:protein will be 1:1.

Optimized for use with IVIS™ fluorescence imaging systems with expanded wavelength capabilities, IVISense dyes enable quantitative imaging of up to four channels and four different biomarkers of interest in vivo. Unit size 1 mg.

IVISense™ 750 fluorescent dyes are ideal for molecular labeling for use in preclinical in vivo imaging applications due to enhanced tissue penetration at this wavelength. IVISense 750 dyes are well suited for the labeling and imaging of proteins, antibodies, and small molecules in a wide range of research areas. The 750 nm fluorescence wavelength is ideal for biological imaging applications using in vivo fluorescence systems but will only work on microscopes, high content imaging and flow cytometry systems that have appropriate light sources and filter sets to accommodate this wavelength.

• Uniquely optimized for the generation of in vivo imaging probes with low non-specific accumulation at tumor sites and inflammatory sites.
• Need special microscopes, flow cytometers, and plate readers that accommodate excitation and emission with light sources and filters for this wavelength.
• Suitable for in vivo multiplexing with 645 or 680 nm Revvity probes or other labeled proteins/Abs.  Expanded in vivo multiplexing with IVIS spectral unmixing with 645/680/800 nm probes or antibodies.
• No spectral interference with mouse diets, so alfalfa-containing mouse diets can be used.
• Recommended dye:protein ratio of 1-3.