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Tag-lite Dopamine D2 Receptor Frozen & Labeled Cells, 200 Assay Points

Name: Tag-lite Dopamine D2 Receptor Frozen & Labeled Cells, 200 Assay Points
Price: 1073.26 USD
Availability: InStock

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Diagram of Dopamine D2 receptor labeled with Tb (transiently expressing cells) image

Tag-lite cells transiently expressing the Dopamine D2 receptor labeled with Terbium for use in receptor binding applications. The cells are provided ready to use.

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Feature	Specification
Application	Receptor-Ligand Binding

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Tag-lite cells transiently expressing the Dopamine D2 receptor labeled with Terbium for use in receptor binding applications. The cells are provided ready to use.

View product information

Product Variant

Unit Size: 200 assay points

Part #:

C1TT1D2

List Price

USD 1,073.26

Your online price:

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).

Total list price:

USD 1,073.26

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Overview

Dopaminergic neurons and dopamine receptors are the most numerous in brain. In the brain, dopamine receptors D1 and D2 are GPCR responsible for such crucial processes as voluntary movement, sleep, regulation of food intake, attention, learning, and working memory.

Cells expressing the D2 receptor are provided pre-labeled with Terbium, and can be used to conduct receptor binding studies on the aforementioned receptor.

Specifications

Other Specifications

Application	Receptor-Ligand Binding
Brand	Tag-lite
Detection Modality	HTRF
Product Group	Cells
Shipping Conditions	Shipped in Dry Ice
Target Class	GPCR
Technology	TR-FRET
Therapeutic Area	Cardiovascular Infectious Diseases Metabolism/Diabetes NASH/Fibrosis Neuroscience Oncology & Inflammation Rare Diseases
Unit Size	200 assay points

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How it works

Assay principle

Running your D2 receptor binding assay using Tag-lite is as easy as it can gets. Simply dispense 10 µL of labeled cells into each well, followed by 5 µL of labeled ligand and 5 µL of the compound you wish to test. Like all HTRF assays, Tag-lite assays do not require any washing steps. A diagram of the procedure to be followed is given on the right.

Saturation binding (KD)

A saturation binding assay measures total and non-specific binding for increasing concentrations of ligand under equilibrium conditions. To perform the assay, the fluorescent ligand is titrated into a solution containing a fixed amount of labeled cells and then incubated to equilibrium. The HTRF ratio obtained from this titration is the total binding.

Competitive binding (KI)

A competitive binding assay is performed to measure the dissociation constant, Ki. To perform the assay, the compound is titrated into a solution containing a fixed concentration of fluorescent ligand and a fixed amount of cells.