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HTRF Streptavidin Eu-Conjugate, 5,000 Assay Points

Streptavidin-Eu cryptate image
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HTRF Streptavidin Eu-Conjugate, 5,000 Assay Points
Streptavidin-Eu cryptate image
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Streptavidin-Eu cryptate image
Streptavidin-Eu cryptate
Streptavidin-Eu cryptate image
Streptavidin-Eu cryptate image
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Eu cryptate-labeled Streptavidin for capturing biotinylated proteins, peptides, antibodies, small molecules, nucleic acids.

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Feature Specification
Application Protein-Protein Interaction
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Eu cryptate-labeled Streptavidin for capturing biotinylated proteins, peptides, antibodies, small molecules, nucleic acids.

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Product Variants
Unit Size: 5,000 assay points
Part #:
610SAKLA
List Price
USD 504.99
Your online price:
Unit Size: 20,000 assay points
Part #:
610SAKLB
List Price
USD 1,616.14
Your online price:
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
Total list price:
USD 504.99
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Streptavidin-Eu cryptate image
Streptavidin-Eu cryptate image
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HTRF Streptavidin Eu-Conjugate, 5,000 Assay Points
Streptavidin-Eu cryptate image
Video Play Button
Streptavidin-Eu cryptate image
Streptavidin-Eu cryptate
HTRF Streptavidin Eu-Conjugate, 5,000 Assay Points
Streptavidin-Eu cryptate image
Streptavidin-Eu cryptate image
Video Play Button

Product information

Overview

Streptavidin has been labeled with Eu crypate. Biotin binds to Streptavidin with high affinity (Ka=1015M-1). The binding is rapid and stable, making it an ideal choice for use in a variety of assays such as enzyme assays, protein-protein binding assays and molecular biology assays.

Specifications

Application
Protein-Protein Interaction
Brand
HTRF
Detection Modality
HTRF
Product Group
Fluorescent Reagent
Shipping Conditions
Shipped Ambient
Target Class
Binding Assay
Technology
TR-FRET
Therapeutic Area
Cardiovascular
Infectious Diseases
Inflammation
Metabolism/Diabetes
NASH/Fibrosis
Neuroscience
Oncology & Inflammation
Rare Diseases
Unit Size
5,000 assay points

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HTRF Streptavidin Eu-Conjugate, 5,000 Assay Points
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HTRF Streptavidin Eu-Conjugate, 5,000 Assay Points
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How it works

Assay principle

In an HTRF interaction assay, one partner is labeled (directly or indirectly) with the donor, and the other with the acceptor (again, directly or indirectly). The intensity of the signal is proportional to the binding of the 2 partners. In the example shown here: Streptavidin-Eu cryptate binds to the biotinylated tagged partner A while partner B* binds to a specific Ab labeled with an HTRF acceptor. *partner B can also be tagged, Fc fused. In these cases use for the detection, the corresponding HTRF reagent (anti-Tag, anti-species, protA, Streptavidin), labeled with acceptor.

Assay principle htrf streptavidin-eu cryptate
Assay protocol

The example on the right describes the protocol using a 20 µL final assay volume for detecting an interaction between a biotinylated-tagged partner A and a non-tagged partner B*. Dispense the 2 partners (10 µL), incubate, add Streptavidin-Eu cryptate (5 µL) and anti-partner B labeled with acceptor (5 µL), incubate and read. *partner B can also be tagged, Fc fused or directly labeled. In these cases use for the detection, the corresponding HTRF reagent (anti-Tag, anti species, protA, Streptavidin), labeled with donor.

Assay protocol htrf streptavidin-eu cryptate

Assay details

How do the number of tests relate to active moiety?

The average conjugate quantity per well reflects overall biological material content. Using the active moiety amount is generally preferred to the quantity of total conjugate. For Cryptate and d2 conjugates, the total conjugate amount equals that of the active moiety, since the molecular weight of the label is negligible. This is not the case for XL665 labeled entities for which the quantity of total conjugate will vary depending on the final molar ratio of the XL665 conjugate, however, the amount of active moiety, provided by Revvity, is constant and based on the number of tests ordered.

Assay specifications
Description Active Moiety/5,000 tests
Streptavidin K 9 µg

 

Recommended quantities of Cryptate and XL665 conjugates

Cryptate conjugates must not be excessive in order to prevent reader saturation and an unacceptable level of background. In most cases, a cryptate concentration of 1 to 5nM is appropriate, and will generate 20,000 to 80,000 cps at 620 nm depending on the HTRF compatible reader used. The XL665 conjugate must match its assay counterpart as closely as possible in order for the maximum number of biomolecules to be tagged with the XL665 acceptor. Thus, to detect a tagged molecule at an assay concentration of 20nM, the concentration of anti-Tag-XL665 should be equimolar or higher.

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