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HTRF Rabbit IgG-Fc Detection Kit, 500 Assay Points

The Rabbit Fc kit is designed for the fast quantification of rabbit IgG or rabbit Fc tagged proteins.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Feature Specification
Application Protein Quantification
Sample Volume 10 µL

The Rabbit Fc kit is designed for the fast quantification of rabbit IgG or rabbit Fc tagged proteins.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Click to copy promo code to clipboard.
SAVE 15% NOW on online orders. Use promo code below.
YEAREND15
Offer valid until 12/15. Terms and conditions apply.
Product Variants
Unit Size: 500 Assay Points
Part #:
6FRIGPEG
List Price
USD 1,086.00
Unit Size: 10,000 Assay Points
Part #:
6FRIGPEH
List Price
USD 12,280.00

Overview

Drug discovery and bioprocess often require accurate quantification technologies to improve the productivity workflow. Current methods such as ELISA do not properly fit with such objectives as they require multiple sample-handling and protocol steps. The Rabbit Fc kit enables a rapid quantification of rabbit IgG and any proteins containing a rabbit Fc region. The assay is compatible with natural IgG as well as recombinant antibodies.

Specifications

Application
Protein Quantification
Brand
HTRF
Detection Modality
HTRF
Product Group
Kit
Sample Volume
10 µL
Shipping Conditions
Shipped in Dry Ice
Target Class
Biologics
Target Species
Rabbit
Technology
TR-FRET
Therapeutic Area
Cardiovascular
Infectious Diseases
Metabolism/Diabetes
NASH/Fibrosis
Neuroscience
Rare Diseases
Unit Size
500 Assay Points

Video gallery

How it works

Assay principle

The rabbit Fc kit is a competitive immunoassay for the measurement of rIgGs or any rFc-tagged proteins in a range of biological materials. The assay uses an Eu3+-Cryptate conjugated antibody specific to rFc and XL665 coupled rIgGs. rFc contained in the sample competes with XL665-labelled rabbit IgG for binding to anti-rabbit Fc Eu Cryptate.

Biomarkers assay principle rabbit
Assay Protocol

The rabbit Fc assay features a streamlined protocol with only 1 incubation step after sample and Rabbit Fc detection reagents dispensing. This protocol requires a single two-hour incubation period at RT.

Biomarkers assay protocol rabbit

 

Assay details

Key features
  Rabbit Fc
Detection limit: 5 ng/mL
Dynamic range: 4.9 80,000 ng/mL
Incubation time: 2h at RT
EC50: 178 ng/mL

 

Specifications rabbit

 

Resources

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Guide
HTRF solutions, guide to major applications

This guide provides you an overview of HTRF applications in several therapeutic areas.

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