The Rabbit Fc kit is designed for the fast quantification of rabbit IgG or rabbit Fc tagged proteins.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
Feature | Specification |
---|---|
Application | Protein Quantification |
Sample Volume | 10 µL |
The Rabbit Fc kit is designed for the fast quantification of rabbit IgG or rabbit Fc tagged proteins.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
Drug discovery and bioprocess often require accurate quantification technologies to improve the productivity workflow. Current methods such as ELISA do not properly fit with such objectives as they require multiple sample-handling and protocol steps. The Rabbit Fc kit enables a rapid quantification of rabbit IgG and any proteins containing a rabbit Fc region. The assay is compatible with natural IgG as well as recombinant antibodies.
Application |
Protein Quantification
|
---|---|
Brand |
HTRF
|
Detection Modality |
HTRF
|
Product Group |
Kit
|
Sample Volume |
10 µL
|
Shipping Conditions |
Shipped in Dry Ice
|
Target Class |
Biologics
|
Target Species |
Rabbit
|
Technology |
TR-FRET
|
Therapeutic Area |
Cardiovascular
Infectious Diseases
Metabolism/Diabetes
NASH/Fibrosis
Neuroscience
Rare Diseases
|
Unit Size |
500 Assay Points
|
The rabbit Fc kit is a competitive immunoassay for the measurement of rIgGs or any rFc-tagged proteins in a range of biological materials. The assay uses an Eu3+-Cryptate conjugated antibody specific to rFc and XL665 coupled rIgGs. rFc contained in the sample competes with XL665-labelled rabbit IgG for binding to anti-rabbit Fc Eu Cryptate.
The rabbit Fc assay features a streamlined protocol with only 1 incubation step after sample and Rabbit Fc detection reagents dispensing. This protocol requires a single two-hour incubation period at RT.
Rabbit Fc | |
---|---|
Detection limit: | 5 ng/mL |
Dynamic range: | 4.9 80,000 ng/mL |
Incubation time: | 2h at RT |
EC50: | 178 ng/mL |
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This guide provides you an overview of HTRF applications in several therapeutic areas.
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