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HTRF PPI Low Ionic Strength Europium Detection buffer, 220 mL
HTRF PPI Low Ionic Strength Europium Detection buffer, 220 mL
Picture of PPI low ionic strength Eu detection buffer
PPI Low Ionic Strength Europium Detection buffer is a spare part intended for use in protein/protein interaction assays.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
| Feature | Specification |
|---|---|
| Application | Protein-Protein Interaction |
PPI Low Ionic Strength Europium Detection buffer is a spare part intended for use in protein/protein interaction assays.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
Product Variant
Unit Size: 220 mL
Part #:
61DB11RDF
List Price
USD 545.00
HTRF PPI Low Ionic Strength Europium Detection buffer, 220 mL
Picture of PPI low ionic strength Eu detection buffer
HTRF PPI Low Ionic Strength Europium Detection buffer, 220 mL
Product information
Overview
This Low Ionic Strength Eu detection buffer is specifically designed for
protein-protein interaction studies. It has been optimized for maximum
performance when the studied protein-protein interaction involves a Europium
cryptate conjugate in the assay and is sensitive to high ionic strength.
Specifications
| Application |
Protein-Protein Interaction
|
|---|---|
| Brand |
HTRF
|
| Detection Modality |
HTRF
|
| Product Group |
Buffer
|
| Shipping Conditions |
Shipped Ambient
|
| Target Class |
Binding Assay
|
| Technology |
TR-FRET
|
| Unit Size |
220 mL
|
Assay validation
Evaluation of the two PPI Eu buffers in protein/protein interaction assays
The two Europium PPI detection buffers were evaluated in two different Protein/Protein interaction assays.
The IC50 values corresponding to the inhibition of the two interactions (A/B & C/D) remained comparable in both PPI Eu detection buffers.
The Protein A/ Protein B interaction displayed the same Signal to Background (S/B) in the PPI Eu detection buffer and the PPI Low Ionic Strength Eu detection buffer.
However, the S/B of the Protein C/ Protein D interaction was significantly improved in the new PPI Low Ionic Strength Eu detection buffer, suggesting that the PPI Low Ionic Strength Eu detection buffer can maximize assay performance when the studied protein-protein interaction is sensitive to high ionic strength.
Resources
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Technical Note
How to access true Kd values in Protein-Protein Interaction assays
Investigate the dynamics of Interactions
Kinetic values are of importance for characterizing protein-protein interactions...
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