A fast and easy way to identify new binders to MDM2 protein.

MDM2, also known as murine double minute 2, is involved in many biological processes and is closely associated with DNA repair, cell cycle arrest, apoptosis, and the occurrence of many diseases. MDM2 is one of the most popular E3 ligases which is recruited by bifunctional Proteolysis-targeting chimeras (PROTACs) to induce ubiquitination and subsequent proteasomal degradation of a targeted protein. MDM2 interacts with several proteins to form the functional E3 ubiquitin ligase complex, in which MDM2 functions as a substrate receptor of the E3 ubiquitin ligase complex and targets various proteins to proteolysis.

Therefore, identifying new PROTAC MDM2 ligands can improve selective proteasomal-dependent degradation of proteins of interest, involved in disease onset such as cancers, neurodegenerative, and metabolic disorders.