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HTRF Human IgG Detection Kit, 500 Assay Points

The Human IgG kit is designed for the fast quantification of human IgG.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Feature	Specification
Application	Protein Quantification
Sample Volume	10 µL

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The Human IgG kit is designed for the fast quantification of human IgG.

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Product Variants

Unit Size: 500 Assay Points

Part #:

6FHIGPEG

List Price

USD 778.36

Unit Size: 10,000 Assay Points

Part #:

6FHIGPEH

List Price

USD 5,350.00

Product information

Overview

Drug discovery and bioprocess often require accurate quantification technologies to improve the productivity workflow. Current methods such as ELISA do not properly fit with such objectives as they require multiple sample-handling and protocol steps. The human IgG kit enables a rapid quantification of human IgG in cell supernatant and the selection of the best producer clones. The assay is compatible with natural IgG as well as recombinant antibodies.

Specifications

Other Specifications

Application	Protein Quantification
Brand	HTRF
Detection Modality	HTRF
Product Group	Kit
Sample Volume	10 µL
Shipping Conditions	Shipped in Dry Ice
Target Class	Biologics
Target Species	Human
Technology	TR-FRET
Therapeutic Area	Cardiovascular Infectious Diseases Metabolism/Diabetes NASH/Fibrosis Neuroscience Rare Diseases
Unit Size	500 Assay Points

Video gallery

How it works

Assay principle

This kit is a sandwich immunoassay enabling highly sensitive quantification of human IgG, even when using a small sample volume. The assay uses two polyclonal antibodies, one (Fc specific) labelled with Cryptate and the second (FAb specific) with d2. The signal intensity is proportional to the number of antibody-antibody complexes formed and therefore to the human IgG concentration.

Assay Protocol

The human IgG assay features a streamlined protocol with only 1 incubation step after sample and human IgG detection reagents dispensing. This protocol requires a single two-hour incubation period at RT.

Assay details

Key features

The figure below shows the human IgG kit calibration curve. The assay was performed in a 384-well small volume white microplate in a 20 µL total volume, using 10µL human IgG calibrator diluted in PBS 0.1 M and 5 µL of each conjugate. The two HTRF conjugates may be pre-mixed and added in a single dispensing step to further streamline the protocol. After 2h of incubation, the signal was read on a PHERAstarFS flash lamp.