Europium cryptate-NH2 labeling reagent enables Europium cryptate labeling of biomolecules.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
Feature | Specification |
---|---|
Application | Labeling |
Europium cryptate-NH2 labeling reagent enables Europium cryptate labeling of biomolecules.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
Europium cryptate-NH2, is a do-it-yourself solution for labeling peptides, oligonucleotides or proteins with Europium cryptate.This reagent is ideal for experienced users familiar with labeling and chromatography. Used in combination with bi-functional reagents such as carbodiimides and SMCC/SPDP. Maleimide group generated by SMCC activation of the Europium cryptate-NH2 reacts with the thiol generated by SPDP/DTT treatment on the biomolecules to label.
Application |
Labeling
|
---|---|
Brand |
HTRF
|
Detection Modality |
HTRF
|
Product Group |
Fluorescent Reagent
|
Shipping Conditions |
Shipped in Dry Ice
|
Technology |
TR-FRET
|
Unit Size |
100 µg
|
As shown here, preactivation of both the Europium cryptate and the biomolecule may be required. Recovery of labeled material can be achieved using conventional chromatographic methods, such as gel filtration or HPLC. The purification devices required for recovering labeled material are not delivered with these reagents.
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