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HTRF d2 Labeling Kit
Second Part Of 62d2dpea
For research use only. Not for use in diagnostic procedures.
Product information
Overview
The d2 labeling kit is suitable for quick, easy labeling of peptides, proteins, and oligonucleotides with d2.
This kit is designed for very small-volume fast labeling needs, such as determining the best antibody among several antibodies available for HTRF assay development.
The N-hydroxysuccinimide activated d2 supplied reacts with primary amines carried by the biomolecules to label, for simplified conjugation.
The kit includes all the materials necessary for the labeling procedure (except dialysis and concentration devices).
Specifications
| Assay Points |
1
|
|---|---|
| Assay Target Type |
Kit
|
| Assay Technology |
HTRF
|
| Brand |
HTRF
|
| Quantity |
1
|
| Unit Size |
1 Vial
|
Video gallery
How it works
Assay principle
The labeling procedure is performed under very mild conditions. As shown here, the process comprises an incubation step with the desiccated dye, followed by purification on a disposable column. Both the purification column and the buffer for purifying the conjugate are included in the kit, as well as a calibrator to define the ideal working dilution of the labeled material in HTRF assays. Depending on the molecular weight of the target molecule and the nature of the dye, each kit enables the labeling of 5-25 µg (protein-MW 15 kDa) to 50-250 µg (antibody-MW 150 kDa).
Assay protocol
The labeling procedure is described on the right. 100 µL of the targeted molecule (16.67 µM) in carbonate buffer pH 9.0 are added to the d2 vial. After an incubation of 45 minutes at RT, labeled protein is eluted on an equilibrated column. After having discarded 700 µL of elution buffer, the labeled molecule is collected in 400µL fraction.
Resources
Guide
HTRF solutions, guide to major applications
This guide provides you an overview of HTRF applications in several therapeutic areas.
SDS, COAs, Manuals and more
Are you looking for technical documents for this product. We have housed them in a dedicated section., click on the links below to explore.
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