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HTRF cAMP Gs HiRange Detection Kit, 1,000 Assay Points

This HTRF kit detects changes of cAMP accumulation in response to Gs coupled GPCR activation or inhibition

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Feature Specification
Application Second Messenger Detection
Sample Volume 10 µL

This HTRF kit detects changes of cAMP accumulation in response to Gs coupled GPCR activation or inhibition

Product Variants
Unit Size: 1,000 Assay Points
Part #:
62AM6PEB
List Price
USD 859.13
Unit Size: 20,000 Assay Points
Part #:
62AM6PEC
List Price
USD 5,350.00

Overview

Based on HTRF technology, the kit contains all the reagents needed to quantify cAMP, ensuring that you benefit from a complete tool for your needs.

Revvity's HTRF cAMP kits are best suited for measuring cAMP modulations in response to Gs-activation, leveraging an optimized combination of robustness and sensitivity

Specifications

Application
Second Messenger Detection
Brand
HTRF
Detection Modality
HTRF
Product Group
Kit
Sample Volume
10 µL
Shipping Conditions
Shipped Ambient
Target Class
GPCR
Technology
TR-FRET
Therapeutic Area
Cardiovascular
Infectious Diseases
Metabolism/Diabetes
NASH/Fibrosis
Neuroscience
Oncology & Inflammation
Rare Diseases
Unit Size
1,000 Assay Points

Video gallery

How it works

Assay principle

Revvity cAMP kits are based on a competitive format involving a specific antibody labeled with cryptate (donor) and cAMP coupled to d2 (acceptor). This enables the direct characterization of all types of compounds acting on Gs-coupled receptors in either adherent or suspension cells.

GPCR how it works assay principle camp gs hirange
Assay protocol

The protocol is simple and straightforward, with just two incubation steps: - Cell stimulation by the target compounds - cAMP detection using HTRF reagents.

GPCR how it works assay protocol camp gs hirange 62am6peb

 

Assay details

cAMP Gs hirange product specifications

If your experimental design uses membrane preparations, lipoparticles or purified receptors, or if your samples have large quantities of cAMP, the Gs HiRange kit will fit your needs!

The kit shows no ATP cross-reactivity up to 10mM, allowing the user to operate with large ranges of ATP and enabling membrane work.

Designed for optimal signal performance, cAMP - Gs HiRange offers a maximized signal-to-background score.

 

Specifications camp gs hirange 1

 

  S/B IC20 nM IC50 nM IC80 nM
cAMP - Gs dynamic >14 0.91 4.07 18.1
cAMP - Gs HiRange >28 4.27 22.8 121

 

ATP cross-reactivity
specifications-camp-gs-hirange-2.svg
Specifications camp gs hirange 3
Specifications camp gs hirange 4

 

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SDS, COAs, Manuals and more

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