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Glycan Profiling Assay Reagent Kit
Glycan Profiling Assay Reagent Kit
Shipping box for Revvity reagent kits
Revvity’s LabChip® Glycan Profiling Assay Reagent Kit enables the high-throughput sizing and quantification of N-linked glycans from protein biotherapeutics on the LabChip® GXII Touch™ protein characterization system.
For research use only. Not for use in diagnostic procedures.
| Feature | Specification |
|---|---|
| Instrument Compatibility | LabChip GXII Touch HT |
| Percent Purity Reproducibility | LMW Protein 30% CV ≤ 80 kDa, HT Glycan: CV < 10% for a peak ≥ 2.5% of total glycans, HT Glycan Extended Range: CV < 10% for a peak ≥ 2.5% of total glycans and at a conc. 2.5 ng/L CV < 25% for a peak ≥ 2.5% of total glycans at conc. 1.0 - 2.5 ng/µL |
| Sample Volume | 8µL with concentration range of 1.25 - 7.5 mg/mL (10 - 60 µg of MAb total) |
| Size Range | HT Glycan: neutral glycans found on mAbs, some charged glycans may run outside of our usable range HT Glycan Extended Range: neutral and charged glycans found on mAbs |
| Sizing Precision RSD (CV) | CV < 2.5% |
Revvity’s LabChip® Glycan Profiling Assay Reagent Kit enables the high-throughput sizing and quantification of N-linked glycans from protein biotherapeutics on the LabChip® GXII Touch™ protein characterization system.
For research use only. Not for use in diagnostic procedures.
Product Variant
Sample Type: Glycan
Part #:
760525
List Price
USD 604.22
Glycan Profiling Assay Reagent Kit
Shipping box for Revvity reagent kits
Glycan Profiling Assay Reagent Kit
Product information
Overview
Glycan profiling and screening, super charged.
A high percentage of all biotherapeutics contain N-linked glycosylation. The impact of N-linked glycosylation on protein function can be significant. Thus, the characterization of N-glycan composition is critical for the biotherapeutic development process. The LabChip® Extended Range N-Linked Glycan assay provides a fast, efficient means to assess the main glycoforms of IgG and related proteins.
Glycoprotein Sizing, Glycoprotein Relative Quantification & Charge Variant N-Glycan Analysis
The LabChip® Glycan Profiling Assay Reagent Kit is ideal for rapid screening of N-linked glycan profiles by combining:
- On-plate protein denaturation
- Rapid enzymatic deglycosylation with PNGase F in 96 well format
- Rapid electrokinetic separations and detection using LabChip® microfluidic chip technology
- Per sample run time is under 45 seconds with a full 96 sample analysis in under 90 minutes
- Automated peak detection and reporting of N-glycan relative abundance
- Minimal sample consumption: Only 8 µL of sample required
- Digital data format: Gel-like image, electropherogram, and tabular data formats with automated sample alignment, overlay, scaling and zooming features, allow users to conveniently share, store and archive data
- The Glycan Profiling Assay Reagent Kit supports both neutral glycan and charged N-linked glycan found in MAbs. Drop down menus allow you to easily choose between assays.
- To release and label glycoforms of IgG and related proteins, use the Glycan Release and Labelling Kit, before analyzing with the Glycan Profiling Assay Reagent Kit
Additional product information
Figure 1: The LabChip Glycan Profiling Assay Reagent Kit supports the sizing and quantification of N-linked glycans in concentrations from 1.25 ng/µl (left) to 7.5 ng/µl (right)
Specifications
| Application |
Protein Analysis
|
|---|---|
| Automation Compatible |
Yes
|
| Instrument Compatibility |
LabChip GXII Touch HT
|
| Percent Purity Reproducibility |
LMW Protein 30% CV ≤ 80 kDa, HT Glycan: CV < 10% for a peak ≥ 2.5% of total glycans, HT Glycan Extended Range: CV < 10% for a peak ≥ 2.5% of total glycans and at a conc. 2.5 ng/L CV < 25% for a peak ≥ 2.5% of total glycans at conc. 1.0 - 2.5 ng/µL
|
| Sample Type |
Glycan
|
| Sample Volume |
8µL with concentration range of 1.25 - 7.5 mg/mL (10 - 60 µg of MAb total)
|
| Size Range |
HT Glycan: neutral glycans found on mAbs, some charged glycans may run outside of our usable range HT Glycan Extended Range: neutral and charged glycans found on mAbs
|
| Sizing Precision RSD (CV) |
CV < 2.5%
|
| Shipping Conditions |
Shipped in Blue Ice Soft
|
| Technology |
Microfluidic Electrophoresis
|
| Unit Size |
1 unit
|
References
- Loebrich, S., Clark, E., Ladd, K., Takahashi, S., Brousseau, A., Kitchener, S., Herbst, R., & Ryll, T. (2018). Comprehensive manipulation of glycosylation profiles across development scales. MAbs, 11(2), 335–349. doi.org/10.1080/19420862.2018.1527665.
Resources
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Product Note
Automated characterization of proteins and nucleic acids
Read the LabChip® GXII Touch™ protein characterization system product note to learn about an automated alternative to traditional...
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