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Glycan Profiling Assay Reagent Kit

Shipping box for Revvity reagent kits
Glycan Profiling Assay Reagent Kit
Shipping box for Revvity reagent kits
Shipping box for Revvity reagent kits
Shipping box for Revvity reagent kits
Shipping box for Revvity reagent kits
Shipping box for Revvity reagent kits

Revvity’s LabChip® Glycan Profiling Assay Reagent Kit enables the high-throughput sizing and quantification of N-linked glycans from protein biotherapeutics on the LabChip® GXII Touch™ protein characterization system.

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Feature Specification
Instrument Compatibility LabChip GXII Touch HT
Percent Purity Reproducibility LMW Protein 30% CV ≤ 80 kDa, HT Glycan: CV < 10% for a peak ≥ 2.5% of total glycans, HT Glycan Extended Range: CV < 10% for a peak ≥ 2.5% of total glycans and at a conc. 2.5 ng/L CV < 25% for a peak ≥ 2.5% of total glycans at conc. 1.0 - 2.5 ng/µL
Sample Volume 8µL with concentration range of 1.25 - 7.5 mg/mL (10 - 60 µg of MAb total)
Size Range HT Glycan: neutral glycans found on mAbs, some charged glycans may run outside of our usable range HT Glycan Extended Range: neutral and charged glycans found on mAbs
Sizing Precision RSD (CV) CV < 2.5%
Technology Microfluidic Electrophoresis
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Revvity’s LabChip® Glycan Profiling Assay Reagent Kit enables the high-throughput sizing and quantification of N-linked glycans from protein biotherapeutics on the LabChip® GXII Touch™ protein characterization system.

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Product Variant
Sample Type: Glycan
Part #:
760525
List Price
USD 623.32
Your online price:
For research use only. Not for use in diagnostic procedures.
Total list price:
USD 623.32
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Shipping box for Revvity reagent kits
Shipping box for Revvity reagent kits
Glycan Profiling Assay Reagent Kit
Shipping box for Revvity reagent kits
Shipping box for Revvity reagent kits
Shipping box for Revvity reagent kits
Glycan Profiling Assay Reagent Kit
Shipping box for Revvity reagent kits
Shipping box for Revvity reagent kits

Supporting products you might need

Chip needed to run Glycan Profiling Assay
Add to bundleView details
LabChip Protein Microfluidics Chip

High Resolution LabChip GXII, Touch HT

Part #: 760524
List Price: USD 1,446.61
Your online price:Log in to view
Add to bundleView details
LabChip Protein Microfluidics Chip

High Resolution LabChip GXII, Touch 24

Part #: CLS138951
List Price: USD 886.56
Your online price:Log in to view
Instrument needed to run Glycan Profiling Assay
Add to bundleView details
LabChip GX and GXII Analyzers

LabChip GXII Touch HT Protein Characterization System

Part #: CLS138160
List Price: USD 169,900.00
Your online price:Log in to view
Optional Glycan Release and Labeling kit
Add to bundleView details
Shipping box for Revvity reagent kits

Glycan Profiling Assay Release and Labeling Kit

Part #: CLS155434
List Price: USD 2,317.89
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Product information

Overview

Glycan profiling and screening, super charged.

A high percentage of all biotherapeutics contain N-linked glycosylation. The impact of N-linked glycosylation on protein function can be significant. Thus, the characterization of N-glycan composition is critical for the biotherapeutic development process. The LabChip® Extended Range N-Linked Glycan assay provides a fast, efficient means to assess the main glycoforms of IgG and related proteins.

Glycoprotein Sizing, Glycoprotein Relative Quantification & Charge Variant N-Glycan Analysis

The LabChip® Glycan Profiling Assay Reagent Kit is ideal for rapid screening of N-linked glycan profiles by combining:

  • On-plate protein denaturation
  • Rapid enzymatic deglycosylation with PNGase F in 96 well format
  • Rapid electrokinetic separations and detection using LabChip® microfluidic chip technology
  • Per sample run time is under 45 seconds with a full 96 sample analysis in under 90 minutes
  • Automated peak detection and reporting of N-glycan relative abundance
  • Minimal sample consumption: Only 8 µL of sample required
  • Digital data format: Gel-like image, electropherogram, and tabular data formats with automated sample alignment, overlay, scaling and zooming features, allow users to conveniently share, store and archive data
  • The Glycan Profiling Assay Reagent Kit supports both neutral glycan and charged N-linked glycan found in MAbs. Drop down menus allow you to easily choose between assays.
  • To release and label glycoforms of IgG and related proteins, use the Glycan Release and Labelling Kit, before analyzing with the Glycan Profiling Assay Reagent Kit

Additional product information

labchip extended range n glycan assay image 1
labchip extended range n glycan assay fig1

Figure 1: The LabChip Glycan Profiling Assay Reagent Kit supports the sizing and quantification of N-linked glycans in concentrations from 1.25 ng/µl (left) to 7.5 ng/µl (right)

Specifications

Application
Protein Analysis
Automation Compatible
Yes
Instrument Compatibility
LabChip GXII Touch HT
Percent Purity Reproducibility
LMW Protein 30% CV ≤ 80 kDa, HT Glycan: CV < 10% for a peak ≥ 2.5% of total glycans, HT Glycan Extended Range: CV < 10% for a peak ≥ 2.5% of total glycans and at a conc. 2.5 ng/L CV < 25% for a peak ≥ 2.5% of total glycans at conc. 1.0 - 2.5 ng/µL
Sample Type
Glycan
Sample Volume
8µL with concentration range of 1.25 - 7.5 mg/mL (10 - 60 µg of MAb total)
Size Range
HT Glycan: neutral glycans found on mAbs, some charged glycans may run outside of our usable range HT Glycan Extended Range: neutral and charged glycans found on mAbs
Sizing Precision RSD (CV)
CV < 2.5%
Shipping Conditions
Shipped in Blue Ice Soft
Technology
Microfluidic Electrophoresis
Unit Size
1 unit

References

  • Loebrich, S., Clark, E., Ladd, K., Takahashi, S., Brousseau, A., Kitchener, S., Herbst, R., & Ryll, T. (2018). Comprehensive manipulation of glycosylation profiles across development scales. MAbs, 11(2), 335–349. doi.org/10.1080/19420862.2018.1527665.

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Automated characterization of proteins and nucleic acids

Read the LabChip® GXII Touch™ protein characterization system product note to learn about an automated alternative to traditional...

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