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GrowDex 2.5 ml

GrowDex® is an animal-free hydrogel that enables complete control over the culture environment for cells.

GrowDex can be used in a wide range of cell-based assays such as microscopic imaging or metabolic activity measurement. GrowDex has been validated on various cell models and applications including primary hepatocytes, patient derived cells, 3D organoid formation, hepatocyte toxicity, stem cell proliferation and differentiation, tumour cell migration and invasion, neurite outgrowth and network formation.

For research use only. Not for use in diagnostic procedures.

GrowDex® is an animal-free hydrogel that enables complete control over the culture environment for cells.

GrowDex can be used in a wide range of cell-based assays such as microscopic imaging or metabolic activity measurement. GrowDex has been validated on various cell models and applications including primary hepatocytes, patient derived cells, 3D organoid formation, hepatocyte toxicity, stem cell proliferation and differentiation, tumour cell migration and invasion, neurite outgrowth and network formation.

For research use only. Not for use in diagnostic procedures.

Product Variants
Quantity: 1 x 2.5 mL
Part #:
100103002
List Price
USD 160.89
Quantity: 1 x 5 mL
Part #:
100103005
List Price
USD 212.79
Quantity: 1 x 10 mL
Part #:
100103010
List Price
USD 387.17

Overview

GrowDex has been designed to provide a support matrix for the culture of cells in a wide range of applications. It comprises of two components, nanofibrillar cellulose (NFC) and ultra-pure water. The length of the nanofibrils ranges from 200-300 μm, with a diameter in the nanometer scale varying from 5-100 nm.

The product is supplied sterilized, ready to use and can be utilized in a variety of cell culture applications, such as 3D spheroid or organoid formation.

Benefits:

  • Animal-free: No animal biomolecules interfering with readouts.
  • Lot to lot reproducibility: nanofibrillar cellulose and purified water only. GrowDex hydrogel is manufactured to the highest standards with strict quality control criteria.
  • Reliable: robust and sustained performance regardless of the production lot ordered.
  • Stable: used and stored at room temperature, GrowDex hydrogel can be used in liquid handling systems.
  • Ready-to-use: simplified protocol in which GrowDex hydrogel is mixed with media and cells.
  • Tunable: Optimal gel stiffness is achieved by dilution with cell culture media. Matrix composition can be optimized easily by supplementing cell culture media with specific biomolecules such as growth factors.
  • Compatibility with High Content Screening systems: no auto-fluorescence and transparency.

Storage and shelf-life:

  • Storage: Ambient
  • Shelf life: 12 months
  • Shelf life after opening: 3 months

Concentration

  • GrowDex is supplied at a concentration of 1.5%.
  • Working concentrations are usually between 0.2% and 1.0%.

GrowDase™ enzyme breaks down GrowDex hydrogel to form a solution in an easy one step efficient cell recovery process. 3D cell structures such as spheroids, organoid or biopsies are retained with no impact on cell viability or functionality. Liberated cells can be used in gene or protein expression studies or for future experiments.

Specifications

Concentration
GrowDex is supplied at a concentration of 1.5%<br>Working concentrations are usually between 0.2% and 1.0%
Form
Hydrogel
Brand
GrowDex®
Quantity
1 x 2.5 mL
Shipping Conditions
Shipped Ambient
Storage Conditions
Ambient

Resources

Are you looking for resources, click on the resource type to explore further.

13-18 of 22 Resources
Application Note Icon
Application Note
GrowDex® Supports Human iPSCderived Neural Progenitor Differentiation in vitro

Here we establish methodologies for 3D cell culture of human iPSC-derived neural progenitors within nanofibrillar cellulose (NFC)...

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Application Note
GrowDex® Supports Robust Human Neuronal Network Formation in vitro

In this study pre-differentiated human pluripotent stem cell derived neurons where encapsulated within GrowDex and cultured.

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Guide
Guide to reducing the cost and time to set-up reproducible HTS/HCS with 3D cell models

Download our guide to reducing the costs and time required to set up reproducible high-throughput and high-content screens using...

Application Note Icon
Application Note
Guidelines for using GrowDex® in cell-based assays

Here we provide guidelines for using GrowDex® in cell-based assays.

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Application Note
High Throughput Cytotoxicity Testing with HepG2 Cells Grown in 3D Culture

Here we have started addressing some of these challenges using GrowDex® hydrogel as a 3D cell culture matrix for growing liver...

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Guide
How to transfer from animal-derived matrices to GrowDex - 3D cell culture matrix

Animal-derived matrices for 3D cell culture are often poorly defined and suffer from lot-to-lot variability. This variability...

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