Fluorescently-labeled Fluorescein-12-CTP nucleotide analog for enzymatic incorporation, for direct fluorescence detection.
For research use only. Not for use in diagnostic procedures.
This fluorescein labeled analog of cytidine triphosphate (CTP) is used for labeling RNA probes via in vitro transcription with various RNA polymerases. Fluorescein labeled RNA probes are used in a variety of molecular biology applications including in situ hybridization and gene expression microarrays. Labeled products may be detected directly via fluorescence or indirectly by combining with anti-fluorescein HRP or AP conjugates.
The number “12” in the compound name refers to the length of the linker arm between CTP and the label.
Concentration |
1 mM
|
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Volume |
25.0 µL
|
Buffer |
10 mM Tris-HCl, pH 7.6, 1 mM EDTA
|
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Detection Method |
Direct
|
Fluorophore |
Fluorescein
|
Nucleotide |
CTP
|
One Unit Contains |
25.0 nmol
|
Shipping Conditions |
Shipped Ambient
|
Unit Size |
25 nmol
|
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