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DELFIA Streptavidin Eu-Conjugate, 250 µg

DELFIA® Europium-labeled streptavidin, for use in DELFIA time-resolved fluorescence (TRF) assays.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Feature Specification
Application Protein-Protein Interaction

DELFIA® Europium-labeled streptavidin, for use in DELFIA time-resolved fluorescence (TRF) assays.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Product Variant
Unit Size: 250 µg
Part #:
1244-360
List Price
USD 540.24

Overview

DELFI® Eu-labeled streptavidin is intended for use as a generic reagent to detect biotin-labeled compounds, e.g. antibodies or antigens in various types of immunoassay or nucleic acid probes in hybridization assays based on solid-phase separation. The label, Eu3+, is measured using dissociation-enhanced time-resolved fluorometry.

The streptavidin is isolated from fermentation filtrates of Streptomyces avidinii and 1 mg of streptavidin binds approximately 10 μg of biotin. The preparation is supplied ready to use in 50 mmol/L Tris-HCl buffered saline solution. For DELFIA immunoassays, a final concentration of 100 ng/mL is recommended for the generation of a suitable signal.

Specifications

Application
Protein-Protein Interaction
Automation Compatible
Yes
Brand
DELFIA
Conjugates
Streptavidin
Detection Modality
DELFIA
Fluorophore
Eu-N1
Product Group
Fluorescent Reagent
Shipping Conditions
Shipped in Blue Ice
Target Class
Binding Assay
Technology
TRF
Unit Size
250 µg

Citations

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Application Note
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DELFIA® immunoassays are particularly well suited for discovery of specific, high-affinity monoclonal antibodies (mAbs)...

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Application Note
Optimization and miniaturization of DELFIA TRF assays converted from ELISA.

This detection method is similar to a traditional ELISA, however DELFIA’s utilization of lanthanide metals endows DELFIA with...

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