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DELFIA Eu-N1 Streptavidin

DELFIA® Europium-labeled streptavidin, for use in DELFIA time-resolved fluorescence (TRF) assays.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Part number: 1244-360
List price: USD 540.24
Your price:
USD 540.24
USD 540.24 /each

Overview

DELFI® Eu-labeled streptavidin is intended for use as a generic reagent to detect biotin-labeled compounds, e.g. antibodies or antigens in various types of immunoassay or nucleic acid probes in hybridization assays based on solid-phase separation. The label, Eu3+, is measured using dissociation-enhanced time-resolved fluorometry.

The streptavidin is isolated from fermentation filtrates of Streptomyces avidinii and 1 mg of streptavidin binds approximately 10 μg of biotin. The preparation is supplied ready to use in 50 mmol/L Tris-HCl buffered saline solution. For DELFIA immunoassays, a final concentration of 100 ng/mL is recommended for the generation of a suitable signal.

Specifications

Antibody Conjugates
Streptavidin
Application
Protein-protein interactions
Protein Analysis & Detection
Automation Compatible
Yes
Brand
DELFIA
Detection Method
DELFIA TRF
Time-Resolved Fluorescence (TRF)
Experimental Type
In vitro
Fluorophore
Eu-N1
Format
Microplates
One Unit Contains
250.0 µg
Shipping Conditions
Shipped in Blue Ice
Unit Size
250 µg

Citations

Resources

1-2 of 2 Resources
Application Note Icon
Application Note
DELFIA Assays: Flexible and Sensitive Tools for Monoclonal Antibody Development

DELFIA® immunoassays are particularly well suited for discovery of specific, high-affinity monoclonal antibodies (mAbs)...

Application Note Icon
Application Note
Optimization and miniaturization of DELFIA TRF assays converted from ELISA.

This detection method is similar to a traditional ELISA, however DELFIA’s utilization of lanthanide metals endows DELFIA with...

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