DELFIA® Europium-labeled anti-human IgG, for use in DELFIA time-resolved fluorescence (TRF) immunoassays.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
DELFIA® Eu-Labeled Anti-Human IgG is intended for use as a secondary antibody in various types of immunoassay based on solid-phase separation. The label, Eu3+, is measured using dissociation-enhanced time-resolved fluorometry.
The products are made from in vitro produced and affinity-purified mouse monoclonal IgG2b antibody specific for the Fc-part of human IgG. The antibodies recognize all IgG subclasses and cross-react with rabbit immunoglobulins.
This product is supplied ready-to-use in 50 mmol/L Tris-HCl buffered saline solution. As a general guideline for DELFIA® immunoassays, a final concentration of 100 ng/mL of the anti-human IgG antibodies may be suitable for generation of the signal.
Antibody Conjugates |
Anti-human IgG
|
---|---|
Application |
Protein-protein interactions
Protein Analysis & Detection
|
Automation Compatible |
Yes
|
Brand |
DELFIA
|
Detection Method |
DELFIA TRF
Time-Resolved Fluorescence (TRF)
|
Experimental Type |
In vitro
|
Fluorophore |
Eu-N1
|
Format |
Microplates
|
One Unit Contains |
100.0 µg
|
Shipping Conditions |
Shipped in Blue Ice
|
Unit Size |
100 µg
|
DELFIA® immunoassays are particularly well suited for discovery of specific, high-affinity monoclonal antibodies (mAbs)...
DELFIA immunoassays are a superior alternative to traditional ELISAs
This guide provides an introduction to the DELFIA method, and to the labeling procedures involved.
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