Rabbit polyclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects human MEK1 phosphorylated at Ser217 and Ser221. It also reacts with MEK1 singly phosphorylated at Ser217 or Ser221. MEK1 is phosphorylated in vivo by RAF1 and COT. Homologous phosphorylation sites are found in MEK2. Sequence motif recognized: GQLIDSMANSFVG
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
LANCE Ultra time-resolved fluorescence resonance energy transfer (TR-FRET) assays use a proprietary europium chelate donor dye, W1024 (Eu), together with ULight™, a small molecular weight acceptor dye with a red-shifted fluorescent emission. Validated for over 310 kinases, we provide data transparency by supplying:
For this antibody:
In this kinase assay configuration, the phosphorylated biotinylated substrate is detected by both the ULight-labeled streptavidin and Eu-labeled anti-phosphosubstrate antibodies, which brings donor and acceptor dyes into close proximity. After irradiation of the kinase reaction at 320 or 340 nm, the energy from the Eu donor is transferred to the ULight acceptor dye which, in turn, generates light at 665 nm. The intensity of the light emission is proportional to the level of ULight substrate phosphorylation.
Antibody Conjugates |
Anti-phospho-MEK1/2
|
---|---|
Application |
Kinase
|
Automation Compatible |
Yes
|
Brand |
LANCE Ultra
|
Detection Method |
TR-FRET
|
Fluorophore |
Eu-W1024
|
Format |
Microplates
|
Molecular Modification |
Phosphorylation
|
One Unit Contains |
10.0 µg
|
Shipping Conditions |
Shipped in Blue Ice
|
Therapeutic Area |
Autoimmunity
Immuno-oncology
Metabolic
Neuroscience
Oncology
|
Unit Size |
10 µg
|
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