Europium-labeled monoclonal antibody recognizing human Histone H4 (H4Kac pan). For use in LANCE® Ultra epigenetic writer and eraser assays.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
The LANCE® Ultra Europium-anti-acetyl-Histone H4 Lysine (H4Kac pan) antibody was used for the development and optimization of a TIP60 acetylation assay using a biotinylated Histone H4 peptide as substrate. A technical note describing the assay is available in our product literature.
Formats:
Proven, cost-efficient LANCE Ultra reagents can be used to quantitate peptide modifications, detecting specific meythylation and acetylation states. No wash, homogenous LANCE Ultra reagents are HTS friendly - design your own epigenetics screening strategy for greatest efficiency.
Epigenetic enzymatic assays are optimized using a biotinylated histone H3-derived peptide as substrate. The modified peptide is captured by the Eu-labeled antibody (Ab) and ULight-Streptavidin (SA) which bring the Eu donor and ULight acceptor dye molecules into close proximity. Upon irradiation at 320 or 340 nm, the energy from the Eu donor is transferred to the ULight acceptor dye which, in turn, generates light at 665 nm. The intensity of the light emission is proportional to the level of biotinylated substrate modification.
Application |
Biochemical Enzymatic Assay
|
---|---|
Automation Compatible |
Yes
|
Brand |
LANCE Ultra
|
Conjugates |
Anti-acetyl-lysine
|
Detection Modality |
LANCE
|
Fluorophore |
Eu-W1024
|
Molecular Modification |
Acetylation
|
Product Group |
Antibody
|
Shipping Conditions |
Shipped in Dry Ice
|
Target Class |
Epigenetics
|
Technology |
TR-FRET
|
Unit Size |
100 µg
|
LANCE™ Ultra TR-FRET is a no-wash proximity assay technology that combines the benefits of time resolution (TR) with fluorescence...
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