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DELFIA Yellow Plate, Yellow 96-well MicroPlate, 60 plates

96-well yellow microplates with high protein bind treatment, for coating with your own biomolecule-of-interest for use in DELFIA time-resolved fluorescence assays.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Part number: AAAND-0001
Unit Size: Case of 60
List price: USD 624.44
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USD 624.44
USD 624.44 /each

Overview

DELFIA® Yellow plates have a high protein binding capacity combined with exceptionally low fluorescence background. The lower background and, hence, increased sensitivity result from
a UV-absorbing agent in the plastic material that prevents the excitation of the plastic. The properties of the yellow plate are especially beneficial in multi-analyte DELFIA® assays where samarium or terbium is used as a second label alongside europium.

DELFIA® (dissociation-enhanced lanthanide fluorescence immunoassay) is a time-resolved fluorescence (TRF) intensity technology. Assays are designed to detect the presence of a compound or biomolecule using lanthanide chelate labeled reagents, separating unbound reagent using wash steps. DELFIA assays are flexible, compatible with a variety of plate readers, and, as this is a wash-based technology, compatible with most sample types. The technology is based on fluorescence of lanthanide chelates (Europium, Samarium, and Terbium). The fluorescence decay time of these lanthanide chelate labels is much longer than traditional fluorophores, allowing efficient use of temporal resolution for reduction of autofluorescent background. The large Stokes’ shift (difference between excitation and emission wavelengths) and the narrow emission peaks contribute to increasing signal-to-noise ratio. Sensitivity is further increased because of the dissociation-enhancement principle: the lanthanide chelate is dissociated and a new highly fluorescent chelate is formed into a protective micellar solution. DELFIA lanthanide chelates require this dissociation/enhancement step for fluorescence (induced by addition of DELFIA Enhancement solution, DELFIA Inducer, and DELFIA Enhancer as appropriate to the particular lanthanide chelate).

Specifications

Color
Yellow
Well Density
96 wells
Well Shape
Flat-bottom
Application
Research
Automation Compatible
Yes
Brand
DELFIA
Coating Treatment
High-binding surface
Detection Method
Time-Resolved Fluorescence (TRF)
Format
Microplates
Lids Included?
No
Material
Polystyrene
Shipping Conditions
Shipped Ambient
Sterility
Non-Sterile
Unit Size
Case of 60

Resources

1-2 of 2 Resources
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eBook
Guide to Selecting a Microplate

Your specific applications deserve the best microplates!

For a researcher, the need to select the most suitable application...

Application Note Icon
Application Note
Optimization and miniaturization of DELFIA TRF assays converted from ELISA.

This detection method is similar to a traditional ELISA, however DELFIA’s utilization of lanthanide metals endows DELFIA with...

SDS, COAs, Manuals and more

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