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  • Home
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  • Immunoassays
  • DELFIA
  • DELFIA Tb-N1-ITC Chelate and Terbium (Tb) Standard, 1mg

DELFIA Tb-N1-ITC Chelate and Terbium (Tb) Standard, 1mg

generic vial
DELFIA Tb-N1-ITC Chelate and Terbium (Tb) Standard, 1mg
generic vial
generic vial
generic vial

Terbium labeling chelate for creating your own Terbium-labeled biomolecules for use in DELFIA time-resolved fluorescence (TRF) assays. The ITC-activated chelate reacts primarily with free amino groups, such as lysines.

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Feature Specification
Application Labeling
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Terbium labeling chelate for creating your own Terbium-labeled biomolecules for use in DELFIA time-resolved fluorescence (TRF) assays. The ITC-activated chelate reacts primarily with free amino groups, such as lysines.

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Product variant
Unit Size: 1 mg
Part #:
AD0009
List price
USD 3,156.35
Your online price:
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For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
Total list price:
USD 3,156.35
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generic vial
DELFIA Tb-N1-ITC Chelate and Terbium (Tb) Standard, 1mg
generic vial
generic vial
DELFIA Tb-N1-ITC Chelate and Terbium (Tb) Standard, 1mg
generic vial
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Product information

  • Overview
  • Specifications

Overview

DELFIA® Tb-N1 ITC chelate is optimized for the Terbium labeling of proteins and peptides for use in dissociation-enhanced time-resolved fluorometric assays.

Isothiocyanate (ITC) activated chelates react primarily with free amino groups on the compound to be labeled.

DELFIA® (dissociation-enhanced lanthanide fluorescence immunoassay) is a time-resolved fluorescence (TRF) intensity technology. Assays are designed to detect the presence of a compound or biomolecule using lanthanide chelate labeled reagents, separating unbound reagent using wash steps. DELFIA assays are flexible, compatible with a variety of plate readers, and, as this is a wash-based technology, compatible with most sample types. The technology is based on fluorescence of lanthanide chelates (Europium, Samarium, and Terbium). The fluorescence decay time of these lanthanide chelate labels is much longer than traditional fluorophores, allowing efficient use of temporal resolution for reduction of autofluorescent background. The large Stokes’ shift (difference between excitation and emission wavelengths) and the narrow emission peaks contribute to increasing signal-to-noise ratio. Sensitivity is further increased because of the dissociation-enhancement principle: the lanthanide chelate is dissociated and a new highly fluorescent chelate is formed into a protective micellar solution. DELFIA lanthanide chelates require this dissociation/enhancement step for fluorescence (induced by addition of DELFIA Enhancement solution, DELFIA Inducer, and DELFIA Enhancer as appropriate to the particular lanthanide chelate).

Specifications

Application
Labeling
Automation Compatible
Yes
Brand
DELFIA
Detection Modality
DELFIA
Fluorophore
Tb-N1-ITC
Product Group
Fluorescent Reagent
Shipping Conditions
Shipped in Blue Ice
Technology
TRF
Unit Size
1 mg

Resources

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  • Application Note (1)
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1-1 of 1 Resources
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Application Note
DELFIA Assays: Flexible and Sensitive Tools for Monoclonal Antibody Development

DELFIA® immunoassays are particularly well suited for discovery of specific, high-affinity monoclonal antibodies (mAbs)...

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