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DELFIA Eu-Labeling Kit (Eu-N1-ITC Chelate), 0.2mg
The DELFIA® Sm-Labeling kit is intended for labeling of proteins with samarium (Sm), for use in multiplexing dissociation enhanced time resolved fluoroimmunoassays.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product information
Overview
Each kit contains 0.2 mg labeling reagent plus Sm-standard, DELFIA Enhancement Solution, Sm-stabilizer, purified BSA for increasing the stability of labeled proteins, an uncoated microtitration strip plate, DELFIA Assay Buffer and DELFIA Wash Concentrate. The kit contents are sufficient for a labeling of up to 1 mg of protein or antibody.
N1 chelates can be used in DELFIA separation assays where the concentration of chelating agents is < 0.05 mmol/L, pH >7, and the temperature is < 38°C. They are suitable for highly sensitive end point measurements in separation based assays such those for detection of protein-protein binding and cell adhesion as well as for immunoassays. Isothiocyanate activated chelates react primarily with free lysines on the compound to be labeled.
Specifications
| Application |
Protein-protein interactions
Protein Analysis & Detection
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
DELFIA
|
| Detection Method |
DELFIA TRF
Time-Resolved Fluorescence (TRF)
|
| Experimental Type |
In vitro
|
| Fluorophore |
Sm-N1-ITC
|
| Format |
Microplates
|
| One Unit Contains |
0.2 mg
|
| Quantity in a Package Amount |
0.02 mg
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Unit Size |
1 Kit
|
Resources
DELFIA immunoassays are a superior alternative to traditional ELISAs
This guide provides an introduction to the DELFIA method, and to the labeling procedures involved.
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