DELFIA® Lysis buffer is intended for use in cell-mediated DELFIA cytotoxicity assay (for the determination of the maximum release). The product forms part of the AD0116 DELFIA® EuTDA Cytotoxicity Reagents pack, and is also available as a separate product.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
DELFIA® time-resolved fluorescence (TRF) cytotoxicity assays offer a non-radioactive method to be used in cell mediated cytotoxicity studies. The method is based on loading cells with an acetoxymethyl ester of a fluorescence enhancing ligand. After the ligand has penetrated the cell membrane the ester bonds are hydrolyzed within the cell to form a hydrophilic ligand, which no longer passes through the membrane. After cytolysis the released ligand is introduced to a europium solution to form a fluorescent chelate. The measured signal correlates directly with the amount of lysed cells.
Application |
Protein Analysis & Detection
|
---|---|
Automation Compatible |
Yes
|
Brand |
DELFIA
|
Detection Method |
DELFIA TRF
Time-Resolved Fluorescence (TRF)
|
Experimental Type |
In vitro
|
Format |
Microplates
|
One Unit Contains |
30.0 mL
|
Shipping Conditions |
Shipped in Blue Ice
|
Unit Size |
30 mL
|
DELFIA® immunoassays are particularly well suited for discovery of specific, high-affinity monoclonal antibodies (mAbs)...
This guide provides an introduction to the DELFIA method, and to the labeling procedures involved.
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