DELFIA Lysis Buffer, 30mL

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DELFIA Lysis Buffer, 30mL

DELFIA^® Lysis buffer is intended for use in cell-mediated DELFIA cytotoxicity assay (for the determination of the maximum release). The product forms part of the AD0116 DELFIA^® EuTDA Cytotoxicity Reagents pack, and is also available as a separate product.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Product information

SDS, COAs, Manuals and more

Product information

Overview

DELFIA® time-resolved fluorescence (TRF) cytotoxicity assays offer a non-radioactive method to be used in cell mediated cytotoxicity studies. The method is based on loading cells with an acetoxymethyl ester of a fluorescence enhancing ligand. After the ligand has penetrated the cell membrane the ester bonds are hydrolyzed within the cell to form a hydrophilic ligand, which no longer passes through the membrane. After cytolysis the released ligand is introduced to a europium solution to form a fluorescent chelate. The measured signal correlates directly with the amount of lysed cells.

Specifications

Other Specifications

Application	Protein Analysis & Detection
Automation Compatible	Yes
Brand	DELFIA
Detection Method	DELFIA TRF Time-Resolved Fluorescence (TRF)
Experimental Type	In vitro
Format	Microplates
One Unit Contains	30.0 mL
Shipping Conditions	Shipped in Blue Ice
Unit Size	30 mL