DELFIA Lysis Buffer, 30mL

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DELFIA Lysis Buffer, 30mL

SDS

TDS

DELFIA^® Lysis buffer is intended for use in cell-mediated DELFIA cytotoxicity assay (for the determination of the maximum release). The product forms part of the AD0116 DELFIA^® EuTDA Cytotoxicity Reagents pack, and is also available as a separate product.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Product Variant

Unit Size: 30 mL

Part #:

4005-0010

List Price

USD 362.95

Product information

Overview

DELFIA® time-resolved fluorescence (TRF) cytotoxicity assays offer a non-radioactive method to be used in cell mediated cytotoxicity studies. The method is based on loading cells with an acetoxymethyl ester of a fluorescence enhancing ligand. After the ligand has penetrated the cell membrane the ester bonds are hydrolyzed within the cell to form a hydrophilic ligand, which no longer passes through the membrane. After cytolysis the released ligand is introduced to a europium solution to form a fluorescent chelate. The measured signal correlates directly with the amount of lysed cells.