DELFIA® Inducer is used to create a fluorescent Europium or Samarium chelate for measuring DELFIA time-resolved fluorescence (TRF) assays. This buffer is an alternative to Enhancement Solution for Eu and/or Sm measurements.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
DELFIA® Inducer is a ready-for-use solution with Triton X-100, glycine, hydrochloric acid and chelators. It is intended for use in the rapid quantitative determination of Eu3+ and Sm3+ in time-resolved fluorometric assays. It is suitable for use in multilabel assays that require DELFIA® Enhancer for development of Tb3+ and Dy3+ fluorescence. The solution dissociates lanthanide from solid phase bound DELFIA® chelate labeled biomolecules in a few minutes depending from used chelate to form a homogeneous and highly fluorescent Eu/Sm-(BFPP)3(TOPO)2-3 micellar chelate solution. The solution allows highly sensitive Eu3+/Sm3+ measurements to be performed when using a time-resolved fluorometer.
For use with stable chelates such as DTPA, to provide more rapid signal development. Suitable for use in all multianalyte assays.
Assay Technology |
TRF
|
---|---|
Automation Compatible |
Yes
|
Brand |
DELFIA
|
Detection Method |
DELFIA
|
Product Group |
Buffer
|
Unit Size |
250 mL
|
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This guide provides an introduction to the DELFIA method, and to the labeling procedures involved.
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