DELFIA® Europium labeling kit for labeling your own biomolecules for use in DELFIA time-resolved fluorescence assays. The ITC chelate reacts with free amine groups in the target biomolecule.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
The DELFIA Eu-N1 ITC chelate is optimized for the europium labeling of proteins and peptides for use in dissociation-enhanced time-resolved fluorometric assays. Isothiocyanate (ITC) activated chelates react primarily with free amine groups such as those at the N-terminus of protein or lysine residues.
Each kit contains 0.4 mg labeling reagent plus Eu-standard, DELFIA Enhancement Solution, Eu-stabilizer, purified BSA for increasing the stability of labeled proteins, an uncoated microtitration strip plate, DELFIA Assay Buffer and DELFIA Wash Concentrate. The kit contents are sufficient for a labeling of up to 1 mg of protein or antibody.
Application |
Protein-protein interactions
Protein Analysis & Detection
|
---|---|
Automation Compatible |
Yes
|
Brand |
DELFIA
|
Detection Method |
DELFIA TRF
Time-Resolved Fluorescence (TRF)
|
Experimental Type |
In vitro
|
Fluorophore |
Eu-N1-ITC
|
Format |
Microplates
|
One Unit Contains |
0.2 mg
|
Quantity in a Package Amount |
0.4 mg
|
Shipping Conditions |
Shipped in Blue Ice
|
Unit Size |
1 Kit
|
DELFIA® immunoassays are particularly well suited for discovery of specific, high-affinity monoclonal antibodies (mAbs)...
DELFIA immunoassays are a superior alternative to traditional ELISAs
This guide provides an introduction to the DELFIA method, and to the labeling procedures involved.
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