DELFIA® Enhancement Solution is used to create a fluorescent Europium or Samarium chelate for measuring DELFIA time-resolved fluorescence (TRF) assays. This solution is required for all DELFIA TRF assays.
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The DELFIA® Enhancement Solution is an acidic chelating detergent solution intended for use in the quantitative determination of Eu3+/Sm3+ when using a time-resolved fluoroimmunoassay. The solution dissociates Eu3+/Sm3+ from solid phase bound Eu-labeled antibodies or proteins during a time period of a few minutes to form a homogeneous and highly fluorescent micellar chelate solution. The solution allows highly sensitive Eu3+/Sm3+ measurements to be made when using the time-resolved fluorometer. The DELFIA Enhancement Solution is also used as part of Tb3+/Dy3+ measurement together with DELFIA® Enhancer.
Because DELFIA TRF chelates are not intrinsically fluorescent, this solution (an acidic chelating detergent solution) is added to the microplate wells after a wash step to develop the fluorescence signal. This provides highly sensitive Eu and/or Sm measurements. Suitable for use in all multianalyte assays.
Application |
Protein-protein interactions
Protein Analysis & Detection
|
---|---|
Automation Compatible |
Yes
|
Brand |
DELFIA
|
Detection Method |
DELFIA TRF
Time-Resolved Fluorescence (TRF)
|
Experimental Type |
In vitro
|
Format |
Microplates
|
One Unit Contains |
250.0 mL
|
Unit Size |
250 mL
|
DELFIA® immunoassays are particularly well suited for discovery of specific, high-affinity monoclonal antibodies (mAbs)...
This guide provides an introduction to the DELFIA method, and to the labeling procedures involved.
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