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DELFIA Enhancement Solution, 250mL

DELFIA® Enhancement Solution is used to create a fluorescent Europium or Samarium chelate for measuring DELFIA time-resolved fluorescence (TRF) assays. This solution is required for all DELFIA TRF assays.

All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Part number: 1244-105
List price: USD 322.04
Your price:
USD 322.04
USD 322.04 /each

Overview

The DELFIA® Enhancement Solution is an acidic chelating detergent solution intended for use in the quantitative determination of Eu3+/Sm3+ when using a time-resolved fluoroimmunoassay. The solution dissociates Eu3+/Sm3+ from solid phase bound Eu-labeled antibodies or proteins during a time period of a few minutes to form a homogeneous and highly fluorescent micellar chelate solution. The solution allows highly sensitive Eu3+/Sm3+ measurements to be made when using the time-resolved fluorometer. The DELFIA Enhancement Solution is also used as part of Tb3+/Dy3+ measurement together with DELFIA® Enhancer.

Because DELFIA TRF chelates are not intrinsically fluorescent, this solution (an acidic chelating detergent solution) is added to the microplate wells after a wash step to develop the fluorescence signal. This provides highly sensitive Eu and/or Sm measurements. Suitable for use in all multianalyte assays.

Specifications

Application
Protein-protein interactions
Protein Analysis & Detection
Automation Compatible
Yes
Brand
DELFIA
Detection Method
DELFIA TRF
Time-Resolved Fluorescence (TRF)
Experimental Type
In vitro
Format
Microplates
Unit Size
250 mL

Resources

1-2 of 2 Resources
Application Note Icon
Application Note
DELFIA Assays: Flexible and Sensitive Tools for Monoclonal Antibody Development

DELFIA® immunoassays are particularly well suited for discovery of specific, high-affinity monoclonal antibodies (mAbs)...

Guide Icon
Guide
Time-resolved fluorometry with the DELFIA method user guide

This guide provides an introduction to the DELFIA method, and to the labeling procedures involved.

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SDS, COAs, Manuals and more

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