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DELFIA Enhancement Solution, 250mL

DELFIA® Enhancement Solution is used to create a fluorescent Europium or Samarium chelate for measuring DELFIA time-resolved fluorescence (TRF) assays. This solution is required for all DELFIA TRF assays.

All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

DELFIA® Enhancement Solution is used to create a fluorescent Europium or Samarium chelate for measuring DELFIA time-resolved fluorescence (TRF) assays. This solution is required for all DELFIA TRF assays.

Product Variant
Unit Size: 250 mL
Part #:
1244-105
List Price
USD 322.04

Overview

The DELFIA® Enhancement Solution is an acidic chelating detergent solution intended for use in the quantitative determination of Eu3+/Sm3+ when using a time-resolved fluoroimmunoassay. The solution dissociates Eu3+/Sm3+ from solid phase bound Eu-labeled antibodies or proteins during a time period of a few minutes to form a homogeneous and highly fluorescent micellar chelate solution. The solution allows highly sensitive Eu3+/Sm3+ measurements to be made when using the time-resolved fluorometer. The DELFIA Enhancement Solution is also used as part of Tb3+/Dy3+ measurement together with DELFIA® Enhancer.

Because DELFIA TRF chelates are not intrinsically fluorescent, this solution (an acidic chelating detergent solution) is added to the microplate wells after a wash step to develop the fluorescence signal. This provides highly sensitive Eu and/or Sm measurements. Suitable for use in all multianalyte assays.

Specifications

Automation Compatible
Yes
Brand
DELFIA
Detection Modality
DELFIA
Product Group
Buffer
Technology
TRF
Unit Size
250 mL

Resources

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Application Note
DELFIA Assays: Flexible and Sensitive Tools for Monoclonal Antibody Development

DELFIA® immunoassays are particularly well suited for discovery of specific, high-affinity monoclonal antibodies (mAbs)...

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Guide
Time-resolved fluorometry with the DELFIA method user guide

This guide provides an introduction to the DELFIA method, and to the labeling procedures involved.

SDS, COAs, Manuals and more

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