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Cyanine 3-ddATP
Fluorescently-labeled Cyanine 3-ddATP nucleotide analog for enzymatic incorporation, for direct fluorescence detection.
For research use only. Not for use in diagnostic procedures.
Product information
Overview
Cyanine 3 labeled dideoxyadenosine triphosphate is biologically active and can be incorporated with a variety of DNA polymerases including Taq, Klenow fragment, and Sequenase™. It terminates further incorporation and enables identification of the complementary base for applications like DNA sequencing and detection of point mutations via single base extension
Specifications
| Concentration |
0.10 mM
|
|---|---|
| Volume |
25.0 µL
|
| Buffer |
10 mM Tris-HCL, pH 7.6, 0.1 mM EDTA
|
|---|---|
| Detection Method |
Direct
|
| Fluorophore |
Cyanine 3
|
| Nucleotide |
ddATP
|
| One Unit Contains |
2.5 nmol
|
| Unit Size |
2.5 nmol
|
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