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chemagic miRNA 1K Kit H24
chemagic miRNA 1K Kit H24
The purification of small RNA species such as microRNA (miRNA) is challenging because of the low concentrations, small fragment sizes (19-22 nucleotides) and tendency to be found in protein complexes. Due to its essential role in homeostasis, miRNA is an important biomarker for cancer and metabolic disease research.
With only 10 minutes of hands-on time within a 90-minute total processing time, the chemagic miRNA kits enable high throughput and efficient extraction of circulating cell-free small RNA from 200 µl or 1 ml inputs in 24-well or 96-well format. The chemagic miRNA kits obtained equivalent yields to manual methods, while analysis by quantitative PCR and Next Generation Sequencing revealed high diversity and reliable performance.
“We have started the testing of cfRNA isolations with our chemagic 360 instrument – the RNA yields appear fine from different plasma and serum samples, thus we look forward to use the kit for high throughput applications.”
Dr. Maija Puhka, Head of HiPrep and EV Core, Institute for Molecular Medicine Finland FIMM, University of Helsinki
For research use only. Not for use in diagnostic procedures.
| Feature | Specification |
|---|---|
| Instrument Compatibility | chemagic™ 360 |
| Nucleic Acid Type | miRNA |
| Sample Type |
Exosomes Plasma Saliva Serum |
The purification of small RNA species such as microRNA (miRNA) is challenging because of the low concentrations, small fragment sizes (19-22 nucleotides) and tendency to be found in protein complexes. Due to its essential role in homeostasis, miRNA is an important biomarker for cancer and metabolic disease research.
With only 10 minutes of hands-on time within a 90-minute total processing time, the chemagic miRNA kits enable high throughput and efficient extraction of circulating cell-free small RNA from 200 µl or 1 ml inputs in 24-well or 96-well format. The chemagic miRNA kits obtained equivalent yields to manual methods, while analysis by quantitative PCR and Next Generation Sequencing revealed high diversity and reliable performance.
“We have started the testing of cfRNA isolations with our chemagic 360 instrument – the RNA yields appear fine from different plasma and serum samples, thus we look forward to use the kit for high throughput applications.”
Dr. Maija Puhka, Head of HiPrep and EV Core, Institute for Molecular Medicine Finland FIMM, University of Helsinki
For research use only. Not for use in diagnostic procedures.
chemagic miRNA 1K Kit H24
chemagic miRNA 1K Kit H24
Product information
Overview
The chemagic miRNA 1k Kit H24 is designed for the purification of circulating small RNA (microRNA) from 1 ml plasma or serum samples, as well as from extracellular vesicles. The kit is designed for consistent and scalable small RNA extraction with comparable yields to manual methods, as well as suitability for various downstream assays.
Specifications
| Application |
DNA/RNA Isolation
Molecular Biology
Next Generation Sequencing
NGS
Research
RNA
|
|---|---|
| Brand |
chemagic
|
| Format |
24 Rod Head
Automated
|
| Instrument Compatibility |
chemagic™ 360
|
| Nucleic Acid Type |
miRNA
|
| Research Areas |
Biobanking
Genomic Analysis
Oncology
|
| Sample Type |
Exosomes
Plasma
Saliva
Serum
|
| Sample Volume |
Up to 1 ml
|
| Technology |
M-PVA Magnetic Bead Technology
|
| Throughput |
Up to 24 samples/run
|
| Type |
microRNA
|
| Unit Size |
240 preps/kits
|
Resources
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For many years it was thought that there were just three types of RNA: mRNA, transfer RNA (tRNA), and ribosomal RNA (rRNA)...
In the rapidly evolving field of molecular biology, the extraction and analysis of small RNA from plasma have become pivotal for...
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