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Celigo Image Cytometer

The Celigo™ image cytometer is a microplate-based multichannel brightfield and fluorescent imaging cytometer for 2D and 3D culture using both adherent and suspension cells. A 21 CFR Part 11 module is available.

For research use only. Not for use in diagnostic procedures.

Overview

Celigo is a plate-based benchtop brightfield and fluorescent imaging system designed for whole-well live-cell analysis and cell sample characterization. The Celigo system images and analyzes cells in various types of vessels including 6 – 1536 well plates, T25, T75 flasks, 10 cm dishes, and glass slides without disturbing their natural state.

Individual cell level analysis is easily generated, providing cell level insights unlike ELISA or protein-based assays, and at a faster rate than flow cytometry. A broad range of complex cell-based assays have been optimized for the Celigo cytometer:

  • Apoptosis
  • Cell cycle
  • Fluorescent reporters
  • Cytotoxicity
  • Label-free proliferation

Rapid whole-well imaging and analysis

  • Sensitive whole-well imaging ensures accurate cell population analysis
    • Remove non-uniform seeding from the equation
    • Accurately detect, image, and count every cell in every well
  • Specialized scanning mirrors enable fast, high-quality images

Advanced brightfield imaging and four fluorescent channels

  • Proprietary algorithm for simultaneous imaging and label-free cell analysis
  • Perform real-time multiplex assays with four fluorescent channels

Convenient workflow designed for biologists

  • Multiple object-driven algorithms and a variety of assay-based applications
    • Accessible adaptation with easy-to-follow software
    • Save customizable experiments as well as default settings
    • Built-in gating parameters allow easy data analysis and visualization
    • Real-time graphic feedback allows multiple parameters to be measured simultaneously for intuitive analysis

Automation and data management

  • Accessible application programming interface (API) for easy automated workflow integration
  • Automated microplate handling for kinetic end-point analysis or time-point analysis

Additional product information

Cell counts with whole-well imaging
celigo diagram

 

Perform brightfield imaging in combination with four fluorescence channels to provide high speed, fully automated imaging and quantification of suspension and adherent cells, tumor spheroids, iPSC, and cancer stem cell colonies.

Proprietary optics and scanning system enable fast imaging of the entire well while maintaining consistent illumination and contrast out to the well edge, for identification of cells within each well.

With an intuitive software interface and optional integration into automation platforms, the Celigo provides labs with increased capabilities. Celigo showcases its value from enabling high-throughput screens of drug libraries in early drug discovery through running high-throughput assays for late-stage research.

Brightfield imaging for all well sizes

Excellent optics for enhanced image quality. Improves brightfield optical image quality at the edge of wells and reduces edge optical distortion by using an F-Theta lens for superior well edge-to-edge image contrast. 

Celigo brightfield imaging
Celigo Imaging Cytometer brightfield image showing the edge of a well on a 96-well microplate showing enhanced image quality and contrast.
celigo bright field imaging
Conventional microscope brightfield image showing distortion and low contrast near the edge of the field-of-view.
Fast plate scanning for image acquisition and analysis

Increase plate-acquisition speed using novel plate scanning technology for fast acquisition. Scan a 384-well plate in brightfield in two minutes or less.

Ability to scan a variety of plate vessels

Scan wells of various sizes using automated image stitching that can view and quantify cells and colonies in vessels up to 6-well plates and 10 cm dishes.

celigo 12 well plate acquisition
celigo 96 well plate

Examples of a 12-well and 96-well plate acquisition using Celigo Imaging Cytometer. 

Quantify cells and colonies

Quantify cells and colonies in the well even if they do not grow uniformly across a well. 

celigo counted cell colonies
Brightfield image of a well on a 96-well microplate showing counted cell colonies across the well.
celigo 96 well microplate showing counted adherent cells
Brightfield image of a well on a 96-well microplate showing counted adherent cells.
Measure adherent cells without trypsinization

Analyze your cell sample without trypsinization to help avoid losing cells and look at cells right where they grow over multiple scan times.

Series of customized applications for each assay - ready-to-use

Label-free brightfield cell analysis: Take advantage of label-free brightfield applications to avoid staining cells with toxic dyes or transfecting with fluorescent reporters.

celigo bright field cell counting growth curve
Label-free brightfield cell counting and from (a) low count to (b) high cell count which can be used to directly generate (c) a cell growth curve.
celigo label free bright field cell counting
Label-free brightfield cell counting of (a) spheroids, (b) cell counting, or (c) confluence percentage.

Numerous cell characterization assays: Live cell analysis of images for cell counting, confluence, colonies, and 3D-spheroids.

Ability to export brightfield and fluorescent images for publications

Capitalize on the Celigo brightfield and fluorescent image quality to take images of your cells for your records and strengthen your publications. 

Easily access and manage data using Celigo network database

Facilitate access to your imaging data using the Celigo network database and connect multiple instruments and satellite workstations to seamlessly acquire and analyze your data from a convenient centralized location.

Easy-to-use preset assay parameters for quick data analysis

Perform Celigo assays using pre-defined parameters that require very few modifications from the researchers. 

Simple and intuitive software user interface

Benefit from a simple and intuitive four-step software workflow to run all your assays. 

Expand your lab's image analysis & data management capabilities

Storing, managing and sharing large volumes of image data can be a challenge. That's why we've made the Signals Image Artist™ image analysis and management platform from Revvity compatible with Celigo image data for use alongside the Celigo system's own powerful acquisition, visualization and analysis software. 

With the addition of the Signals Image Artist platform to your lab, you'll be able to quickly process, store, and share all your image data from the Celigo image cytometer and other major cell imaging systems in a single central location. Its powerful image processing capabilities and ready-made analysis building blocks will provide you with more options for exploring your data and gaining new insights. ​Benefit from machine learning and artificial intelligence (AI) capabilities to train the software to develop analysis algorithms - and get answers faster. You can even access data and perform analysis remotely via the browser login.

*Signals Image Artist platform is sold separately - it is not sold as part of the Celigo system.

Celigo specifications
Software
  • Proprietary image acquisition and processing software
  • Powerful analysis software/Dell Precision computer
  • Windows 10
Illumination/optics
  • 1 LED-based enhanced brightfield imaging channel with uniform well illumination
  • 4 LED-based fluorescent channels
  • Proprietary F-theta lens with superior well edge-to-edge contrast
  • Galvanometric mirrors for fast imaging of large areas
  • Large chip CCD camera (2024 x 2024 pixels)
  • 1, 2, 4 or 8 μm/pixel resolution
Plate compatibility
  • 6, 12, 24 48, 96, 384, 1536 well plates (black, white and clear wall plates)
  • T-25 and T-75 flasks
  • Slides and cell arrays plate profiles available upon request
High-speed imaging
  • Less than 2 minutes per 384-well plate
Weight and dimensions
  • Dimensions: 19.5″W x 16″H x 24″D (49.5cm x 40cm x 61cm)
  • Weight: 117 lbs. (53 kg)
Power requirements
  • 110-220 VAC 50-60 Hz
Regularity compliance
  • CE marking

 

Fluorescent channels
Channel Excitation Dichroic Emission Typical dyes
Blue 377/50 409 470/22 Hoechst, DAPI
Green 483/32 506 536/40 FITC, Calcein, GFP,AlexaFluor® 488
Red 531/40 593 629/53 R-PE, PI, Texas Red, AlexaFluor 568
Far-Red 628/40 660 688/31 DRAQ5®, AlexaFluor 647

Specifications

Brand
Celigo

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