The Celigo™ image cytometer is a microplate-based multichannel brightfield and fluorescent imaging cytometer for 2D and 3D culture using both adherent and suspension cells. A 21 CFR Part 11 module is available.
For research use only. Not for use in diagnostic procedures.
Celigo is a plate-based benchtop brightfield and fluorescent imaging system designed for whole-well live-cell analysis and cell sample characterization. The Celigo system images and analyzes cells in various types of vessels including 6 – 1536 well plates, T25, T75 flasks, 10 cm dishes, and glass slides without disturbing their natural state.
Individual cell level analysis is easily generated, providing cell level insights unlike ELISA or protein-based assays, and at a faster rate than flow cytometry. A broad range of complex cell-based assays have been optimized for the Celigo cytometer:
Rapid whole-well imaging and analysis
Advanced brightfield imaging and four fluorescent channels
Convenient workflow designed for biologists
Automation and data management
Perform brightfield imaging in combination with four fluorescence channels to provide high speed, fully automated imaging and quantification of suspension and adherent cells, tumor spheroids, iPSC, and cancer stem cell colonies.
Proprietary optics and scanning system enable fast imaging of the entire well while maintaining consistent illumination and contrast out to the well edge, for identification of cells within each well.
With an intuitive software interface and optional integration into automation platforms, the Celigo provides labs with increased capabilities. Celigo showcases its value from enabling high-throughput screens of drug libraries in early drug discovery through running high-throughput assays for late-stage research.
Excellent optics for enhanced image quality. Improves brightfield optical image quality at the edge of wells and reduces edge optical distortion by using an F-Theta lens for superior well edge-to-edge image contrast.
Increase plate-acquisition speed using novel plate scanning technology for fast acquisition. Scan a 384-well plate in brightfield in two minutes or less.
Scan wells of various sizes using automated image stitching that can view and quantify cells and colonies in vessels up to 6-well plates and 10 cm dishes.
Examples of a 12-well and 96-well plate acquisition using Celigo Imaging Cytometer.
Quantify cells and colonies in the well even if they do not grow uniformly across a well.
Analyze your cell sample without trypsinization to help avoid losing cells and look at cells right where they grow over multiple scan times.
Label-free brightfield cell analysis: Take advantage of label-free brightfield applications to avoid staining cells with toxic dyes or transfecting with fluorescent reporters.
Numerous cell characterization assays: Live cell analysis of images for cell counting, confluence, colonies, and 3D-spheroids.
Capitalize on the Celigo brightfield and fluorescent image quality to take images of your cells for your records and strengthen your publications.
Facilitate access to your imaging data using the Celigo network database and connect multiple instruments and satellite workstations to seamlessly acquire and analyze your data from a convenient centralized location.
Perform Celigo assays using pre-defined parameters that require very few modifications from the researchers.
Benefit from a simple and intuitive four-step software workflow to run all your assays.
Storing, managing and sharing large volumes of image data can be a challenge. That's why we've made the Signals Image Artist™ image analysis and management platform from Revvity compatible with Celigo image data for use alongside the Celigo system's own powerful acquisition, visualization and analysis software.
With the addition of the Signals Image Artist platform to your lab, you'll be able to quickly process, store, and share all your image data from the Celigo image cytometer and other major cell imaging systems in a single central location. Its powerful image processing capabilities and ready-made analysis building blocks will provide you with more options for exploring your data and gaining new insights. Benefit from machine learning and artificial intelligence (AI) capabilities to train the software to develop analysis algorithms - and get answers faster. You can even access data and perform analysis remotely via the browser login.
*Signals Image Artist platform is sold separately - it is not sold as part of the Celigo system.
Software |
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Illumination/optics |
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Plate compatibility |
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High-speed imaging |
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Weight and dimensions |
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Power requirements |
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Regularity compliance |
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Channel | Excitation | Dichroic | Emission | Typical dyes |
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Blue | 377/50 | 409 | 470/22 | Hoechst, DAPI |
Green | 483/32 | 506 | 536/40 | FITC, Calcein, GFP,AlexaFluor® 488 |
Red | 531/40 | 593 | 629/53 | R-PE, PI, Texas Red, AlexaFluor 568 |
Far-Red | 628/40 | 660 | 688/31 | DRAQ5®, AlexaFluor 647 |
Brand |
Celigo
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