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BCIP/NBT Chromogenic Substrate (2 x 250 mL)
BCIP/NBT substrate detects alkaline phosphatase in blotting and slide applications. Available in ready to use solution, it deposits a permanent dark purple stain on membrane sites bearing phosphatase and with the combination of BCIP(5-bromo-4-chloro-3indolyl-phosphate and NBT(nitrobluetetrazoleum) produces much higher sensitivity than either reagent separately.
For research use only. Not for use in diagnostic procedures.
Product information
Overview
Use enough reagent to completely cover the membrane surface or at least 0.25 ml/cm2 of membrane.
This substrate solution is formulated for use on PolyScreen® PVDF Transfer Membrane, nitrocellulose membranes and the nylon membranes GeneScreen™ Hybridization Transfer Membrane and GeneScreen Plus® Hybridization Transfer Membrane.
Specifications
| Detection Method |
Absorbance/Colorimetric
|
|---|---|
| One Unit Contains |
500.0 mL
|
| Quantity in a Package Amount |
2.0 Units
|
| Shipping Conditions |
Shipped Ambient
|
| Unit Size |
2 x 250 mL
|
Resources
With applications in biotechnology, molecular biology, proteomics, and more, western blotting is the most widely used technique...
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