ATP-monitoring luminescence assay for quantitative evaluation of proliferation and cytotoxicity of cultured mammalian cells.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
Feature | Specification |
---|---|
Application | Cell Viability |
Assay Points | 5000 |
ATP-monitoring luminescence assay for quantitative evaluation of proliferation and cytotoxicity of cultured mammalian cells.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
ATP is a marker for cell viability because it is present in all metabolically active cells. Because ATP concentration declines rapidly when cells undergo necrosis or apoptosis, monitoring ATP is a good indicator of cytocidal, cytostatic and proliferation effects. Discover our ATPlite™ cell viability assay. Our ATPlite and ATPlite 1step luminescence assay systems use patented innovative technologies that measure cell viability, proliferation and cytotoxicity in mammalian cells based on the production of light caused by the reaction of ATP with added luciferase and D-luciferin.
ATPlite is an Adenosine TriPhosphate (ATP) monitoring system based on firefly (Photinus pyralis) luciferase. This luminescence cell viability assay is the alternative to colorimetric, fluorometric and radioisotopic assays for the quantitative evaluation of proliferation and cytotoxicity of cultured mammalian cells. ATP monitoring can be used to assess the cytocidal, cytostatic and proliferative effects of a wide range of drugs, biological response modifiers and biological compounds.
Advantages:
Application |
Cell Viability
|
---|---|
Assay Points |
5000
|
Automation Compatible |
Yes
|
Brand |
ATPlite
|
Cellular or Signaling Pathway |
Autophagy
Cell Death
Cell Growth
Cytotoxicity
Necrosis
|
Cellular Process |
Autophagy
Cell Death
Cell Growth
Cytotoxicity
Necrosis
|
Detection Modality |
Luminescence
|
Format |
Microplates
|
Protocol |
2-step
|
Signal Halflife |
At least 5 hours
|
Shipping Conditions |
Shipped Ambient
|
Unit Size |
5,000 (96-well) / 20,000 (384-well)
|
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