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ATPlite Luminescence Assay System 96-well, 5000 Assay Points

ATP-monitoring luminescence assay for quantitative evaluation of proliferation and cytotoxicity of cultured mammalian cells.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Product Variants
Part number: 6016943
Unit Size: 300 (96-well) / 1200 (384-well)
List price: USD 180.79
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USD 180.79 /each
Part number: 6016941
Unit Size: 1,000 Assay Points (96-well) / 4,000 (384-well)
List price: USD 523.66
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USD 0.00
USD 523.66 /each
Part number: 6016947
Unit Size: 5,000 (96-well) / 20,000 (384-well)
List price: USD 1,734.00
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USD 1,734.00
USD 1,734.00 /each
Part number: 6016949
Unit Size: 10,000 (96-well) / 40,000 (384-well)
List price: USD 3,465.00
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USD 0.00
USD 3,465.00 /each

Overview

ATP is a marker for cell viability because it is present in all metabolically active cells. Because ATP concentration declines rapidly when cells undergo necrosis or apoptosis, monitoring ATP is a good indicator of cytocidal, cytostatic and proliferation effects. Discover our ATPlite™ cell viability assay. Our ATPlite and ATPlite 1step luminescence assay systems use patented innovative technologies that measure cell viability, proliferation and cytotoxicity in mammalian cells based on the production of light caused by the reaction of ATP with added luciferase and D-luciferin.

ATPlite is an Adenosine TriPhosphate (ATP) monitoring system based on firefly (Photinus pyralis) luciferase. This luminescence cell viability assay is the alternative to colorimetric, fluorometric and radioisotopic assays for the quantitative evaluation of proliferation and cytotoxicity of cultured mammalian cells. ATP monitoring can be used to assess the cytocidal, cytostatic and proliferative effects of a wide range of drugs, biological response modifiers and biological compounds.

Advantages:

  • Long-lived luminescent signal: half-life (t1/2) greater than 5 hours, depending on cell type and medium
  • Rapid: results generated in 15 - 25 minutes
  • Simple and reproducible: no separation steps; only two reagent additions
  • Homogeneous assay: no cell harvesting or centrifugation required
  • Sensitive: down to 5 cells in 100µL medium (derived from CHO and HL-60 cells in 100µL medium)
  • Wide linear dynamic range: =105 (as derived from CHO and HL-60 cells)

Specifications

Application
Cell Viability
Assay Pathway
Autophagy
Cell Death
Cell Growth
Cytotoxicity
Necrosis
Assay Points
5000
Assay Protocol
2-step
Automation Compatible
Yes
Brand
ATPlite
Cellular Process
Autophagy
Cell Death
Cell Growth
Cytotoxicity
Necrosis
Detection Method
Luminescence
Format
Microplates
Shipping Conditions
Shipped Ambient
Unit Size
5,000 (96-well) / 20,000 (384-well)

Citations

Resources

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