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ATPlite Luminescence Assay System 96-well, 5000 Assay Points

ATP-monitoring luminescence assay for quantitative evaluation of proliferation and cytotoxicity of cultured mammalian cells.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Feature Specification
Application Cell Viability
Assay Points 5000

ATP-monitoring luminescence assay for quantitative evaluation of proliferation and cytotoxicity of cultured mammalian cells.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Click to copy promo code to clipboard.
Save 25% when you buy this product online. Use promo code below.
LITES25
Offer valid until 12/31. Terms and conditions apply.
Product Variants
Unit Size: 300 (96-well) / 1200 (384-well)
Part #:
6016943
List Price
USD 180.79
Unit Size: 5,000 (96-well) / 20,000 (384-well)
Part #:
6016947
List Price
USD 1,734.00
Unit Size: 10,000 (96-well) / 40,000 (384-well)
Part #:
6016949
List Price
USD 3,465.00

Overview

ATP is a marker for cell viability because it is present in all metabolically active cells. Because ATP concentration declines rapidly when cells undergo necrosis or apoptosis, monitoring ATP is a good indicator of cytocidal, cytostatic and proliferation effects. Discover our ATPlite™ cell viability assay. Our ATPlite and ATPlite 1step luminescence assay systems use patented innovative technologies that measure cell viability, proliferation and cytotoxicity in mammalian cells based on the production of light caused by the reaction of ATP with added luciferase and D-luciferin.

ATPlite is an Adenosine TriPhosphate (ATP) monitoring system based on firefly (Photinus pyralis) luciferase. This luminescence cell viability assay is the alternative to colorimetric, fluorometric and radioisotopic assays for the quantitative evaluation of proliferation and cytotoxicity of cultured mammalian cells. ATP monitoring can be used to assess the cytocidal, cytostatic and proliferative effects of a wide range of drugs, biological response modifiers and biological compounds.

Advantages:

  • Long-lived luminescent signal: half-life (t1/2) greater than 5 hours, depending on cell type and medium
  • Rapid: results generated in 15 - 25 minutes
  • Simple and reproducible: no separation steps; only two reagent additions
  • Homogeneous assay: no cell harvesting or centrifugation required
  • Sensitive: down to 5 cells in 100µL medium (derived from CHO and HL-60 cells in 100µL medium)
  • Wide linear dynamic range: =105 (as derived from CHO and HL-60 cells)

Specifications

Application
Cell Viability
Assay Points
5000
Automation Compatible
Yes
Brand
ATPlite
Cellular or Signaling Pathway
Autophagy
Cell Death
Cell Growth
Cytotoxicity
Necrosis
Cellular Process
Autophagy
Cell Death
Cell Growth
Cytotoxicity
Necrosis
Detection Modality
Luminescence
Format
Microplates
Protocol
2-step
Signal Halflife
At least 5 hours
Shipping Conditions
Shipped Ambient
Unit Size
5,000 (96-well) / 20,000 (384-well)

Citations

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