ATP-monitoring luminescence assay kit for quantitative evaluation of proliferation and cytotoxicity of mammalian cells cultured in 3D spheroids.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
Feature | Specification |
---|---|
Application | Cell Viability |
Assay Points | 300 |
ATP-monitoring luminescence assay kit for quantitative evaluation of proliferation and cytotoxicity of mammalian cells cultured in 3D spheroids.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
ATP is a marker for cell viability because it is present in all metabolically active cells. Because ATP concentration declines rapidly when cells undergo necrosis or apoptosis, monitoring ATP is a good indicator of cytocidal, cytostatic and proliferation effects. ATP assay is key to successfully detect cell viability. Our ATPlite 3D™ and ATPlite 1step 3D luminescence assay systems use patented innovative technologies that measure cell proliferation and cytotoxicity in 3D cultured mammalian cells based on the production of light caused by the reaction of ATP with added luciferase and D-luciferin.
ATPlite 3D is an Adenosine TriPhosphate (ATP) monitoring system based on firefly (Photinus pyralis) luciferase. This ATPlite luminescence assay is the alternative to colorimetric, fluorometric, high content microscopic analysis and radioisotopic assays for the quantitative evaluation of proliferation and cytotoxicity of mammalian cells cultured in 3D spheroids. ATP monitoring can be used to assess the cytocidal, cytostatic and proliferative effects of a wide range of drugs, biological response modifiers and biological compounds.
Advantages:
Kit Components
Storage: Buffer, MCLS and vials should be stored at 4°C. Microplates and TopSeal-A can be stored at Room Temperature.
Application |
Cell Viability
|
---|---|
Assay Points |
300
|
Automation Compatible |
Yes
|
Brand |
ATPlite 3D
|
Detection Modality |
Luminescence
|
Format |
Microplates
|
Protocol |
2-step
|
Signal Halflife |
At least 5 hours
|
Shipping Conditions |
Shipped Ambient
|
Unit Size |
300 Assay Points (96-well)
|
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