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AlphaScreen Anti-FLAG Donor Beads, 25 mg

Alpha Donor beads conjugated to anti-FLAG antibody. This bead can be used to capture FLAG-tagged proteins for Alpha no-wash protein-protein interaction assays and other applications.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Feature Specification
Application Protein-Protein Interaction

Alpha Donor beads conjugated to anti-FLAG antibody. This bead can be used to capture FLAG-tagged proteins for Alpha no-wash protein-protein interaction assays and other applications.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Unit Size: 1 mg
Part #:
AS103D
List Price
USD 757.38
Unit Size: 5 mg
Part #:
AS103M
List Price
USD 2,957.00
Unit Size: 25 mg
Part #:
AS103R
List Price
USD 13,750.00

Overview

Alpha Donor beads conjugated to anti-FLAG antibody. The mouse monoclonal antibody M2 clone recognizes the FLAG sequence at the N-terminus, Met N-terminus, C-terminus, and internal sites. These beads can be used to capture FLAG-tagged antibodies, proteins and peptides, and can be used in conjunction with AlphaScreen, AlphaLISA, or AlphaPlex Acceptor beads to create no-wash assays for:

  • Analyte detection assays
  • Protein-protein interaction assays
  • Protein-DNA interaction assays
  • Protein-RNA interaction assays
  • Protein-small molecule interaction assays
  • Protein detection assays
  • Enzymatic assays

In a typical Alpha assay, 1 mg of Donor beads is sufficient to run 1,000-2,000 wells using a 25 µL reaction volume. Bead concentration can be adjusted for optimal performance.

Specifications

Application
Protein-Protein Interaction
Automation Compatible
Yes
Bead Type or Material
Alpha Donor
Brand
AlphaLISA
Conjugates
Anti-FLAG
Detection Modality
Alpha
Product Group
Beads
Shipping Conditions
Shipped in Blue Ice
Target Class
Binding Assay
Technology
Alpha
Unit Size
25 mg

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Application Note Icon
Application Note
A fast and simple chemiluminescent assay for monitoring of DNA-protein interactions

Electrophoretic Mobility Shift Assay (EMSA) is a standard technique used to study protein-DNA interactions. It is a radioactive...

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Guide
Alpha Protein-Protein Interaction Quick Start Guide

Alpha has been used to study a wide variety of interactions, including protein:protein, protein:peptide, protein:DNA, protein:RNA...

Application Note Icon
Application Note
Biochemical binding ADCC assays utilizing AlphaLISA toolbox reagents for the characterization of hIgGs and FcγR1A

Fc Gamma Receptors (FcγRs) are critical for the function of therapeutic antibodies and mediate many effector functions.

Therapeutic...

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