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3-Amino-3-Deoxydigoxigenin-9-ddGTP
Hapten-labeled 3-Amino-3-Deoxydigoxigenin-9-ddGTP nucleotide analog for enzymatic incorporation, for indirect detection.
For research use only. Not for use in diagnostic procedures.
Product information
Overview
Aminodigoxigenin (DIG) labeled labeled dideoxyguanosine triphosphate is biologically active and can be incorporated with a variety of DNA polymerases including Taq, Klenow fragment, and Sequenase™. It terminates further incorporation and enables identification of the complementary base for applications like DNA sequencing and detection of point mutations. Aminodigoxigenin-labeled products may be detected with anti-DIG antibody conjugates. For the highest sensitivity in detection, we recommend using an anti-DIG-HRP conjugate with tyramide signal amplification (TSA).
The number "9" in the compound name refers to the length of the linker arm between dCTP and the label.
Specifications
| Concentration |
1 mM
|
|---|---|
| Volume |
25.0 µL
|
| Buffer |
10 mM Tris-HCl, pH 7.6, 1 mM EDTA
|
|---|---|
| Detection Method |
Indirect
|
| Hapten |
DIG
|
| Nucleotide |
ddGTP
|
| One Unit Contains |
25.0 nmol
|
| Unit Size |
25 nmol
|
SDS, COAs, Manuals and more
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