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AlphaScreen IgG (Protein A) Detection Kit, 50,000 Assay Points
AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Protein A conjugated AlphaScreen Acceptor beads.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product information
Overview
This bead kit can be used for many applications, including:
- Antibody detection
- Antibody binding
- Binding assays
- Enzymatic assays
In a typical AlphaScreen assay, one biomolecule of interested is biotinylated and associates with the Streptavidin-coated Donor beads. The AlphaScreen Protein A Acceptor beads are used to capture an antibody. The assay is set up to bring the Donor and Acceptor beads are brought into proximity. Excitation of the Donor beads causes the release of singlet oxygen, which diffuses and triggers the emission of light from the Acceptor beads when in proximity. The amount of light is directly proportional to the degree of interaction. This kit contains enough reagents to run 50,000 wells in 384-well format, using a 25 µL reaction volume.
Specifications
| Antibody Conjugates |
Streptavidin/Protein A
|
|---|---|
| Application |
Protein-protein interactions
Protein Analysis & Detection
|
| Assay Points |
50000
|
| Automation Compatible |
Yes
|
| Brand |
AlphaScreen
|
| One Unit Contains |
50000.0 assay points
|
| Species |
Human
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Unit Size |
50,000 Assay Points
|
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