Revvity Sites Globally
Select your location.
*e-commerce not available for this region.
AlphaScreen 6His Nickel Chelate Detection Kit, 500 Assay Points
View All
AlphaScreen 6His Nickel Chelate Detection Kit, 500 Assay Points
AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and nickel chelate (Ni-NTA) AlphaScreen Acceptor beads. This kit contains enough reagents to run 500 wells in 384-well format, using a 25 µL reaction volume.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
| Feature | Specification |
|---|---|
| Application | Protein-Protein Interaction |
AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and nickel chelate (Ni-NTA) AlphaScreen Acceptor beads. This kit contains enough reagents to run 500 wells in 384-well format, using a 25 µL reaction volume.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
AlphaScreen 6His Nickel Chelate Detection Kit, 500 Assay Points
AlphaScreen 6His Nickel Chelate Detection Kit, 500 Assay Points
Product information
Overview
This bead kit can be used for many applications, including:
- Protein-protein interaction assays
- Protein-peptide interaction assays
- His-tagged protein detection and quantitation
- Protein expression
- Binding studies
In a typical AlphaScreen assay, one biomolecule of interested is biotinylated and associates with the Streptavidin-coated Donor beads. The other biomolecule is His-tagged, and associates with the AlphaScreen nickel chelate Acceptor beads. If the two biomolecules bind to each other, the Donor and Acceptor beads are brought into proximity. Excitation of the Donor beads causes the release of singlet oxygen, which diffuses and triggers the emission of light from the Acceptor beads when in proximity. The amount of light is directly proportional to the degree of interaction. Competition formats are also possible, using the biotinylated His peptide probe provided in the kit.
Specifications
| Application |
Protein-Protein Interaction
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaScreen
|
| Conjugates |
Streptavidin/Nickel chelate
|
| Detection Modality |
Alpha
|
| Host Species |
Human
|
| Product Group |
Kit
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target Class |
Binding Assay
|
| Technology |
Alpha
|
| Unit Size |
500 Assay Points
|
Video gallery
AlphaScreen 6His Nickel Chelate Detection Kit, 500 Assay Points
AlphaScreen 6His Nickel Chelate Detection Kit, 500 Assay Points
Citations
Resources
Are you looking for resources, click on the resource type to explore further.
Alpha has been used to study a wide variety of interactions, including protein:protein, protein:peptide, protein:DNA, protein:RNA...
How can we help you?
We are here to answer your questions.
