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AlphaScreen 6His Nickel Chelate Donor Beads, 1 mg

Alpha Donor beads conjugated to nickel chelate. This bead can be used to capture His-tagged proteins for Alpha no-wash protein-protein interaction assays and other applications.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Feature Specification
Application Protein-Protein Interaction

Alpha Donor beads conjugated to nickel chelate. This bead can be used to capture His-tagged proteins for Alpha no-wash protein-protein interaction assays and other applications.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Unit Size: 1 mg
Part #:
AS101D
List Price
USD 757.38
Unit Size: 5 mg
Part #:
AS101M
List Price
USD 2,957.00
Unit Size: 25 mg
Part #:
AS101R
List Price
USD 13,750.00

Overview

Alpha Donor beads coated with nickel chelate. These beads can be used to capture His-tagged proteins and biomolecules, and can be used in conjunction with AlphaScreen, AlphaLISA, or AlphaPlex Acceptor beads to create no-wash assays for:

  • Protein-protein interaction assays
  • Protein detection assays
  • Enzymatic assays

In a typical Alpha assay, 1 mg of Donor beads is sufficient to run 1,000-2,000 wells using a 25 µL reaction volume. Bead concentration can be adjusted for optimal performance.

Specifications

Application
Protein-Protein Interaction
Automation Compatible
Yes
Bead Type or Material
Alpha Donor
Brand
AlphaLISA
Conjugates
Nickel chelate
Detection Modality
Alpha
Product Group
Beads
Shipping Conditions
Shipped in Blue Ice
Target Class
Binding Assay
Technology
Alpha
Unit Size
1 mg

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Application Note Icon
Application Note
A fast and simple chemiluminescent assay for monitoring of DNA-protein interactions

Electrophoretic Mobility Shift Assay (EMSA) is a standard technique used to study protein-DNA interactions. It is a radioactive...

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Guide
Alpha Protein-Protein Interaction Quick Start Guide

Alpha has been used to study a wide variety of interactions, including protein:protein, protein:peptide, protein:DNA, protein:RNA...

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Application Note
Biochemical binding ADCC assays utilizing AlphaLISA toolbox reagents for the characterization of hIgGs and FcγR1A

Fc Gamma Receptors (FcγRs) are critical for the function of therapeutic antibodies and mediate many effector functions.

Therapeutic...

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